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Page 52
Biochemistry & Molecular Biology Journal
ISSN: 2471-8084
Internat i ona l Conference on
Biotechnology, Biomarkers
& Systems Biology
M a r c h 0 4 - 0 5 , 2 0 1 9
Am s t e r d a m , N e t h e r l a n d s
Biotechnology, Biomarkers & Systems Biology 2019
Background:
There are lots of treatments for cancer but always enzymes are the most efficient. Glutamine can be used to support
proliferation in multiple ways. It is a proteinogenic amino acid, and can be act as a nitrogen donor for the synthesis of amino
acids as well as nucleotides biosynthesis in cellular processes. The presence of L-glutaminase has been reported in various
organisms, including animals, plants, and microorganisms except humans. It’s a treatment enzyme for ALL and L-glutaminase
also has proved ineffectual for treatment solid tumours such as breast cancer and colorectal. In following research Yarrowia
yeast glutaminase is used.
Methods:
In this study Raji, MCF7 and A549 cell lines were cultured in RPMI 1640 with 10% FBS and 5% of CO
2
condition. The
cytotoxic effects of L-glutaminase on Raji, MCF7 and A549 cells were studied using MTT assay. Then, flow cytometry assay was
exploited to measure cell death and apoptosis stage.
Results:
MTT assay showed that L-glutaminase significantly inhibited the cell growth. According to the flow cytometry assay
result, the L-glutaminase was able to induce apoptosis in Raji, MCF7 and A549 cell lines. The apoptosis of Raji cells was more
than other cell lines and A549 was more than MCF-7.
Conclusion:
According to our finding, L-glutaminase obtained from Yarrowia, safe yeast could successfully induce apoptosis in
Raji, MCF7 and A549 cell lines. Therefore, it could be used as a novel and safe therapeutic candidate for cancer treatment.
perzhad.mazloumi@gmail.comAnti-cancer effect of L-glutaminase on acute
lymphoblastic leukemia (Raji), breast cancer
(MCF7) and colorectal cancer (A549) cell lines
Elaheh Madadi Hiyagh
1
, Sahand Mazloum Ravasan
1
, Farshad
Darvishi
3
, Ahad Mokhtarzadeh
1
and Behzad Baradaran
1
1
Immunology Research Center-Tabriz University of Medical Sciences, Iran
2
University of Maragheh, Iran
Biochem Mol biol J 2019, Volume:5
DOI: 10.21767/2471-8084-C1-024