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Page 54
Biochemistry & Molecular Biology Journal
ISSN: 2471-8084
Internat i ona l Conference on
Biotechnology, Biomarkers
& Systems Biology
M a r c h 0 4 - 0 5 , 2 0 1 9
Am s t e r d a m , N e t h e r l a n d s
Biotechnology, Biomarkers & Systems Biology 2019
Background:
Cancer is one of the most important problems in the world. Today Enzymes have been intensively studied as a
source of antitumor compounds. L-Asparaginase (L-ASNase) is one of the most therapeutic enzyme which used for the cancer
therapy. The attendance of L-ASNase has been reported in various organisms, containing animals, plants, and microorganisms
(bacteria, fungi, algae, yeast, and actinomycetes) except humans. In this study we used L-ASNase enzyme which isolated from
Yarrowia yeast.
Methods:
In this study, Raji, MCF7 and A549 cell lines were cultured in RPMI 1640 with 10% FBS and 5% of CO2 condition.
Cytotoxic effects of yeasts L-asparaginase was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT)
assay. Then, flow cytometry assay was exploited to measure cell death and apoptosis stage.
Results:
According to our findings, yeasts L-asparaginase can inhibit cell growth in a time and dose dependent manner. Flow
cytometry assay result showed that yeasts L-asparaginase was able to induce apoptosis in Raji, MCF7 and A549 cell lines. The
apoptosis of raji cells is more than other cell lines and A549 is more than MCF-7.
Conclusion:
Our results showed that yeasts L-asparaginase could successfully induce apoptosis in Raji, MCF7 and A549 cell
lines. Therefore, it could be used as a novel and safe therapeutic candidate for cancer treatment.
perzhad.mazloumi@gmail.comYeasts L-asparaginase inhibits cell growth
and induces apoptosis of acute lymphoblastic
leukaemia (Raji), breast cancer (MCF-7) and lung
cancer (A549) cells in in-vitro system
Sahand Mazloum Ravasan
1
, Elaheh Madadi Hiyagh
1
, Farshad
Darvish
2
, Ahad Mokhtarzadeh
1
and Behzad Baradaran
1
1
Immunology Research Center-Tabriz University of Medical Sciences, Iran
2
University of Maragheh, Iran
Biochem Mol biol J 2019, Volume:5
DOI: 10.21767/2471-8084-C1-024