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Page 51
Biochemistry & Molecular Biology Journal
ISSN: 2471-8084
Internat i ona l Conference on
Biotechnology, Biomarkers
& Systems Biology
M a r c h 0 4 - 0 5 , 2 0 1 9
Am s t e r d a m , N e t h e r l a n d s
Biotechnology, Biomarkers & Systems Biology 2019
Objective:
Syncope is a common clinical problem challenging both cardiologists and general practitioners. In our study, we
selected a number of circulating micro-RNAs and vasoactive amine metabolites to be evaluated in attempts to introduce possible
biomarkers for syncope. To the best of our knowledge, this is the first study to assess the changes in mi-RNAs in syncope patients.
Materials & Methods:
Nineteen patients with history of syncope and nineteen sex and age matched healthy controls participated.
A detailed medical history was recorded and cardiovascular examinations followed by head up tilt table testing (HUTT) were
performed. Three blood samples were withdrawn, first one at baseline, second during syncopal attack and third one after 30
minutes from the end of the tilt test. The levels of three circulating microRNAs (miR-210, miR-1 and miR-34a) and three vasoactive
amine metabolites (endothelin-1, copeptin and serotonin) were quantified.
Results:
In Group A, copeptin significantly increased during syncopal attack by 21.7±0.45 pg/mL vs. 4.3±1.209 pg/mL in control
subjects (Group B; P=0.002). Similarly, endothelin-1 values significantly rose by an average of 28±1.25 pg/mL in syncope patients
vs. 3.35±0.75 pg/mL in healthy controls (P<0.001). Serotonin (5-HT) levels were significantly greater during syncope relative to
baseline in HUTT positive patients by 95.89±3.7 pg/mL vs. 9±1.43 pg/ml (p<0.001) in control subjects. In summary, vasoactive
amines increased with a 3-5 fold change in group A, but showed 1-2 fold increase in the control group. In group A, miR-210 has
increased by a mean of 0.6±0.09 (p<0.001) during syncope (95% CI [0.4-0.79]). While miR-34a values increased by mean of
0.89±0.22 during syncope than baseline value (95% CI [0.42, 1.36]) with significant difference of P=0.001. Likewise, miR-1 levels
was elevated by an average of 0.42±0.07 (95% CI [0.26, 0.58]) with a p<0.001 significance. miRNA levels were 3±1 fold higher in
the syncope patients (Group A) than in controls.
Conclusion:
The selected miRNAs and vasoactive amines have a very promising diagnostic and therapeutic potential as
biomarkers in diagnosing syncope.
ahabib@alhyatt.comElevated circulating microRNAs and vasoactive
amine metabolites in neurocardiogenic syncope
patients: a pilot study
Amira S Habib
1
, Hazem M Warda
1, 2
, Ahmed Wagdi
3
, Amany
Elshorbagy
4
and Ahmad R Bassiouny
4
1
Alhyatt Heart and Vascular Center, Egypt
2
Tanta University Hospital, Egypt
3
Institute of Pharmacology and Toxicology-University Medical Center Goettingen, Germany
4
University of Alexandria, Egypt
Biochem Mol biol J 2019, Volume:5
DOI: 10.21767/2471-8084-C1-024