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Biochemistry & Molecular Biology Journal
ISSN: 2471-8084
Internat i ona l Conference on
Biotechnology, Biomarkers
& Systems Biology
M a r c h 0 4 - 0 5 , 2 0 1 9
Am s t e r d a m , N e t h e r l a n d s
Biotechnology, Biomarkers & Systems Biology 2019
T
he progress in industrialization has blessed mankind with a technologically superior lifestyle but poor management of
industrial waste has in turn poisoned nature. One such chemical is carbon tetra chloride (CCl4), which is a potent environmental
toxin emitted from chemical industries and its presence in the atmosphere is increasing at an alarming rate. Presence of
CCl4 in human body is reported to cause liver damage through free radical mediated inflammatory processes. Kupffer cells
present in the liver are potentially more sensitive to oxidative stress than hepatocytes. Kuffer cells produced tumor necrosis
factor-α (TNF-α) in response to reactive oxygen species (ROS), that might further cause inflammation or apoptosis. In this
study hepatoprotective capacity of antioxidant rich extract of Croton bonplandianus Baill. (CBL) was evaluated on CCl4 induced
acute hepatotoxicity in murine model. Hydro-methanolic extract of C. bonplandianus leaf was used for evaluation of free radical
scavenging activity. Liver cells of experimental mice were damaged using CCl4 andsubsequently hepatoprotective potential of
the plant extract was evaluated using series of in-vivo and in-vitro studies. In the hepatoprotective study, silymarin was used as
a positive control. Antioxidant enzymes, pro-inflammatory markers, liver enzymatic and biochemicalparameters were studied to
evaluate hepatoprotective activity of Croton bonplandianus leaf extract. Free radical scavenging activity of CBL extract was also
observed in WRL-68 cell line. The phytochemicals identified by GCMS analysis were scrutinized using in-silico molecular docking
procedure. The results showed that CBL extract have potent free radical scavenging capacity. The biochemical parameters were
over expressed due to CCl4 administration, which were significantly normalized by CBL extract treatment. This finding was also
supported by histopathological evidences showing less hepatocellularnecrosis, inflammation and fibrosis in CBL and silymarin
treated group, compared to CCl4 group. ROS generated due to H2O2 in WRL-68 cell line were normalize in the highest group (200
μg/ml) when compared with control and negative control (CCl4) group. After molecular docking analysis, it was observed that the
compound α-amyrin present in the leaf extract of C. bonplandianus has better potentiality to protect hepatocellular damages than
the standard drug Silymarin. The present study provided supportive evidence that CBL extract possessespotent hepatoprotective
capacity by ameliorating haloalkane induced liver injury in the murine model. The antioxidant and anti-inflammatory activities
also affirm the same. The synergistic effects of the phytochemicals present in CBL are to be credited for all the hepatoprotective
activity claimed above.
somitimmuno@gmail.comAmelioration of CCl4 induced liver injury in swiss
albino mice by antioxidant rich leaf extract of
Croton bonplandianus Baill
Somit Dutta
1
, Arnab Sen
2
and Tapas Kumar Chaudhuri
3
1
First Author Affiliation (University of North Bengal, India)
2
Second Author Affiliation (University of North Bengal, India)
3
Third Author Affiliation (University of North Bengal, India)
Biochem Mol biol J 2019, Volume:5
DOI: 10.21767/2471-8084-C1-024