Dr. Sadhna Joshi
Associate Professor, Department of Molecular Genetics, Department of Lab. Medicine & Pathobiology, Faculty of Medicine, University of Toronto, Canada
Dr. Joshi began research in 1976 at Institut Jacques Monod, Paris, France with the study of the molecular biology of plant viruses. Her main interest has been to define the strategies used for modulation of viral gene expression. She have demonstrated that in order to regulate their gene expression, the Alfalfa mosaic virus (AMV) can make use of a frameshift mechanism and that the Tobacco mosaic virus (TMV) can make use of uncapped RNAs. Using radiolabelling and western blot analysis, She demonstrated that the 30-35 kD non-structural proteins of TMV and AMV are produced early during the virus life cycle. I have also participated in purification and sequencing of a non-suppresser tRNA (a tRNA3Lys) that enabled read-through of a non-stop codon present at the end of an open reading frame encoding the Turnip yellow mosaic virus (TYMV) 150 kD protein. In addition, She developed a tRNAVal-dependent in vitro translation system, and purified the tRNAVal and the tRNAs lacking tRNAVal.
Dr. Joshi main interest has been to define the strategies used for modulation of viral gene expression.