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Tissue Science 2019
June 17-18, 2019
London, UK
Advances in Tissue
Engineering and
Biomaterials Science
13th Edition of International Conference on
Journal of Biomedical Sciences
ISSN: 2254-609X Page 37
J Biomedical Sci 2019, Volume 08
Virtual biopsies of tissues and carcinomas using vibrational
optical coherence tomography
Frederick H. Silver, Michael Richard and Dominick Benedetto
The State University of New Jersey, USA
Neigel Center for Cosmetic and Laser Surgery, USA
Center for Advanced Eye Care, USA
Vibrational optical coherence tomography (VOCT)
is a new technique that combines the imaging
power of optical coherence tomography with the use
of sound to characterize the physical properties of
tissues. This technique has been developed to perform
“virtual” biopsies and biomechanical measurements
on normal and malignant tissues non-invasively and
non-destructively. It has been previously reported that
cutaneous wound healing and the development of
malignant skin lesions are associated with changes
in tissue stiffness. VOCT produces images of groups
of cells as well as biomechanical information in three
dimensions that candistinguishnormal frompathological
tissue. In addition, the biomechanical properties of the
tissue margins can be characterized. The images and
the biomechanical data from measurements made
on different skin lesions and carcinomas together can
help plan surgical interventions and monitor the healing
process of skin lesions. VOCT produces images of
groups of cells as well as measurement of the tissue
resonant frequency in three dimensions which assists in
distinguishing normal from pathological tissue.
We have imaged and studied several types of skin lesions
including a BCC, SCC Actinic Keratosis and a Nevi using
VOCT to evaluate the morphology, stiffness, depth and
margins of these structures. While cellular components
present in skin and carcinomas have resonant
frequencies in the range of 30 to 60 Hz, normal collagen
has a resonant frequency in the range greater than 90
Hz. In comparison, fibrotic collagen is shown to have
resonant frequencies above 150 Hz as does collagen
from skin lesions.
It is concluded that the ratio of the resonant frequency
squared to the tissue thickness obtained from VOCT
can be used to grade the type of tissue response seen.
Further studies are underway to establish the relationship
between tissue stiffness and lesion morphology for
cellular and fibrotic lesions based on the characteristic
ratios of resonant frequency and tissue thickness.