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Tissue Science 2019
June 17-18, 2019
London, UK
Advances in Tissue
Engineering and
Biomaterials Science
13th Edition of International Conference on
Journal of Biomedical Sciences
ISSN: 2254-609X Page 31
M. Basile et al., J Biomedical Sci 2019, Volume 08
Chemotactic properties of human amniotic fluid-derived
stem cells (hAFSCs) in bone healing
M. Basile1,2, S. Novak2, X. Xin2, G. Stati 1 , L. Centurione1, M.A. Centurione3, S.
Sancilio1, P. Vizzarri1, I. Kalajzic2 and R. Di Pietro1
1G. d’Annunzio University of Chieti-Pescara, Italy
2University of Connecticut Health Center, United States
3National Research Council-Pavia, Italy
Current treatment of large bone defects is based
on autologous or allogenic bone grafts that still
have several limitations. In the past few years, human
amniotic fluid stem cells (hAFSCs) were evaluated for
their osteogenic potential in the repair of bone defects
due to the absence of ethic controversy and risk of
teratocarcinoma formation. Thus, the aim of this study
was to investigate the role of hAFSCs in the regeneration
of critical-size bone defects in calvaria mouse model.
For this purpose, we transduced hAFSCs with cherry
red fluorescent protein and used a recipient transgenic
mouse model carrying GFP fluorescent reporter to
follow the fate of hAFSCs transplanted in vivo into
Healos® construct and distinguish donor and host cells
at the implant site. Our results showed that transduced
hAFSCs can be tracked in vivo directly at the site of
transplantation. Cherry red fluorescent hAFSCs were not
present in the implant site after 3 and 6 weeks. Instead,
the presence of a greater number of GFP-positive cells
in the scaffold at the same time-intervals indicates that
hAFSCs can recruit host cells during the repair process.
Moreover, we observed that hAFSCs are able to attract
mouse bone marrow stromal cells (mBMSCs) in vitro,
suggesting a possible chemotactic property of their
releasing soluble factors. These observations help clarify
the role of hAFSCs in bone tissue repair.
Biography
Mariangela Basile graduated in 2015 in Pharmaceutical Chem-
istry and Technology, UdA Chieti, Italy. In 2013 active collab-
oration in conducting scientific research into Department of
Pharmacy, General Pathology Unit, UdAChieti, Italy. Since 2016
PhD student in Translational Medicine, Cell Biology Lab, Dept.
of Medicine and Ageing Sciences, Inst. of Normal HumanMor-
phology, UdA Chieti, Italy. In 2017/2018, research assistant at
Center for Regenerative Medicine and Skeletal Development,
Reconstructive Science Department, UConn Health Center,
Farmington, Connecticut, USA.