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Tissue Science 2019
June 17-18, 2019
London, UK
Advances in Tissue
Engineering and
Biomaterials Science
13th Edition of International Conference on
Journal of Biomedical Sciences
ISSN: 2254-609X Page 32
Radha Santonocito et al., J Biomedical Sci 2019, Volume 08
Tissue bioadhesives: A study on recombinant mussel
protein Pvfp-5ß
Radha Santonocito
National Research Council (CNR), Italy
Many marine organisms rely on natural adhesives to
attach to various surfaces under wet conditions for
their life-cycle, movement and self-defense in aqueous
tidal environments. Mussel adhesive proteins have
received increased attention in recent years for their
potential applications in several fields, such as medicine,
biomaterials and biotechnology being biocompatible and
able to elicit minimal immune response. The Asian green
mussel Perna viridis secretes several byssal plaque
proteins. The Perna viridis foot protein-5β (Pvfp-5β) is
the first protein to initiate interaction with the substrate,
displacing interfacial water molecules before binding to
the surface. Here, we present a study of recombinant
Pvfp-5β, in which we established the first recombinant
expression in E. coli of the protein. We characterized
recombinant Pvfp-5β and showed that, despite the
circular dichroism spectrum with features of a random
coil, the protein is correctly folded as demonstrated by
mass spectrometry and nuclear magnetic resonance.
We evaluated the cell viability and cell adhesion
capacity of Pvfp-5β using NIH-3T3 and HeLa cell lines.
Our results revealed that the protein has no cytotoxic
effect at the investigated protein concentrations and a
good cell adhesion strength on both glass and plastic
plates. Overall, we show that the adhesive properties of
recombinant Pvfp-5βmake it an efficient surface coating
material, suitable for biomedical applications including
regeneration of damaged tissues.
Biography
Dr. Radha Santonocito has completed her master’s degree in
Biodiversity and Evolution at the age of 26 years from Uni-
versity of Palermo. She is a research fellow at the Institute
of Biophysics (IBF) of the National Research Council of Italy
(CNR).