tissue-science-2019-posters-abstracts

14 / 14

Tissue Science 2019
June 17-18, 2019
London, UK
Advances in Tissue
Engineering and
Biomaterials Science
13th Edition of International Conference on
Journal of Biomedical Sciences
ISSN: 2254-609X Page 42
J Biomedical Sci 2019, Volume 08
Flexible electrical stimulation device with chitosan-based
dressing accelerates angiogenesis in diabetic wounds
Xiao-Feng Wang, Qing-Qing Fang, Wan-Yi Zhao and Wei-Qiang Tan
Zhejiang University, China
Diabetic wounds are recalcitrant to treatment and
still affect millions of people worldwide annually.
Vascular lesions caused by hyperglycaemia are known
to severely impair wound healing capabilities in diabetic
patients, revealing the importance of vessel network
establishment promotion for proper repair. Exogenous
electrical stimulation (ES) is a promising physical
treatment of diabetic chronic wounds, because it
could provide a directional vector to stimulate charged
cells involved in wound healing by enhancing cellular
migration. However, uncertainty remains regarding
the best electrical ES parameters for diabetic wounds
and the molecular mechanisms involved in promoting
wound healing. Moreover, the application of ES is also
inconvenient for patients. Here, we show that high
voltage monophasic pulse current stimulation is the
optimal parameter to improve diabetic wound healing
and that surface electrode is better than insertion
electrode for this purpose. In vitro experiments showed
that monophasic pulse current stimulation enhanced the
proliferation and migration capacity of human umbilical
vein endothelial cells and promoted growth factors
released via the Pi3k/Akt and Erk1/2 pathways. In order
to create a more convenient process for the patients and
provide an optimal environment for cell migration, we
used flexible materials and chitosan (good moisture and
antibacterial effects) to create a preliminarily design of
a flexible ES device with chitosan-based dressing, which
was proven to promote the healing of diabetic wounds
through accelerating angiogenesis in vivo. Thus, our work
provides favourableness support for the development
of a more advanced product that may have clinical
application for diabetic chronic wounds in the future.