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Tissue Science 2019
June 17-18, 2019
London, UK
Advances in Tissue
Engineering and
Biomaterials Science
13
th
Edition of International Conference on
Journal of Biomedical Sciences
ISSN: 2254-609X
Page 38
J Biomedical Sci 2019, Volume 08
Polymer based nanostructured membranes obtained via
electrospinning
Gianluca Turco
University of Trieste, Italy
Introduction:
In this work electrospinning (ELS) was used
for the production of polymer based nanostructured
membranes for biomedical applications. Present work
reports the the characterization of polycaprolactone
(PCL) based membranes, implemented with a bioactive
chitosan derivative (CTL) and antibacterial silver
nanoparticles (nAg). Methods: PCL 12%w/V solved in
DCM:DMF7:3waselectrospun for 1hwithacustommade
ELS device. Parameters: 17kV of potential, flow rate of
0.6mL/h, 27G needle, 15cm of distance. Nanofibers were
characterized bymeans of Scanning ElectronMicroscopy
(SEM) and micro-Computed Tomography. Air-plasma
treatment was used to increase the hydrophilicity of the
membrane surface and to adsorb CTL and CTL-nAg. Ag
was quantified with Inductively Coupled Plasma Mass
Spectrometry ICP-MS. Wettability and biocompatibility of
membranes were tested.
Results:
PCL nanostructured membranes produced with
this technique exhibited an average thickness of 215µm
and an average fibre diameter of 600nm. CTL adsorption
was assessed by means of confocal microscopy using
FITC labelled CTL. Contact angle measurements showed
limited wettability of PCL membrane (as prepared), and
increased hydrophilicity of air-plasma and CTL coated
membranes. Biocompatibility test were performed using
MG63 cells cultured on membrane surface. CTL-coated
membranes were able to support cell adhesion and
proliferation. In contrast, both as-prepared membranes
and air-plasma treated membranes exhibited limited cell
adhesion and proliferation.
Discussion and Conclusions:
This work highlighted the
hydrophilicity and biocompatibility of ELS nanostructured
membranes made of PLC and coated with CTL- nAg.
These results are promising for applications in the field
of tissue engineering.
gturco@units.it