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Tissue Science 2019
June 17-18, 2019
London, UK
Advances in Tissue
Engineering and
Biomaterials Science
13
th
Edition of International Conference on
Journal of Biomedical Sciences
ISSN: 2254-609X
Page 39
J Biomedical Sci 2019, Volume 08
LTP modified vascular graft materials for endothelial cells
growth
Komal Vig
1
Kendra Swain
1
, Takura Mlambo
1
, Paul Baker
2
, Bernabe Tucker
2
,
Vinoy Thomas
2
and
Yogesh Vohra
2
1
Alabama State University,USA
2
University of Alabama at Birmingham, USA
C
ardiovascular disease (CVD) is the no. 1 killer in the
world, and is responsible for >17.3 million deaths
every year1. Bypass surgery, using the autologous vein
has been one of the most effective treatments for CVD.
However, more recently vascular grafts have shown great
potential in bypass surgery. Vascular grafts currently
employ a number of scaffold materials such as Dacron
and ePTFE and treatments that mimic the native vessel
wall2-3. These however, does not work well for small
diameter grafts (<6 mm) due to intimal hyperplasia
and thrombosis. In our study we plan to improve the
endothelialization of intimal surface of graft bymodifying
with low temperature plasma (LTP) to increase the cell
attachment/viability and proliferation. The scaffolds were
treated with LTP using Harris Plasma Cleaner system
with air as the feed gas for 30 sec at 45W (HI setting).
X ray photoelectron spectroscopic analyses and contact
angle wettability studies confirmed the introduction of
oxygenated functionalities on the surface and enhanced
hydrophilicity due to the improvement in oxygen content
~1 in the graft surface from LTP air plasma. Scaffolds
were also modified with fibronectin and collagen by
dipping method. Endothelial cell studies by microscopic
and metabolic assays indicated that cell viability
increased in LTP treated scaffolds especially when
treated with protein. Scaffolds treated with fibronectin
or collagen had cells viable for a week compared to
untreated samples. MTT results validated the improved
number of metabolically active viable cells. Work is under
progress to improve cell viability on these scaffolds.
komalvig@alasu.edu