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Tissue Science 2019
June 17-18, 2019
London, UK
Advances in Tissue
Engineering and
Biomaterials Science
13th Edition of International Conference on
Journal of Biomedical Sciences
ISSN: 2254-609X Page 41
J Biomedical Sci 2019, Volume 08
Single cell nano-electroporation to laser induced
photoporation: Novel approaches for cell therapy and
diagnostics
Tuhin Subhra Santra
Indian Institute of Technology Madras, India
The ability to precisely deliver of foreign cargo
into single living cells is of great interest in cell
biology and therapeutics research. Conventional bulk
electroporation is widely used but has been known to
cause high percentage of cell death and require high
voltage sources. Microfluidic electroporation platforms
can provide high delivery efficiency with high cell
viability through better-controlled electric fields applied
to cells. Here we develop micro/nano fabricated single
cell electroporation platforms, which is an efficient and
fast method for multi-nanolocalized single cell nano-
electroporation, where electroporation takes place on a
multiple region of individual single cell membrane using
ITO nano-electrodes array. The gap between two nano-
electrodes are 70 nm with triangle tip diameter of 40
nm, which intense an electric field in a precise region of
single cell membrane to deliver biomolecules with high
transfection efficiency and high cell viability.
On the other hand we developed photoporation based
devices, where nano-second pulse laser is used to
interact with metal or metal nanoparticles and form
plasmonic nanobubbles, which rapidly grew, coalesced
and collapsed to induce an explosion, resulting strong
fluid on the cell membrane. Thus plasma membrane can
disrupt and form transient membrane pores, allowing the
delivery of cargos from outside to inside the cell. Using
both of these techniques we successfully deliver dyes,
DNA, RNA, QDs andnanoparticles, bacteria in cancer cells
as well as stem cell. These new approaches can allow
us to analyse different dyes/biomolecules interaction in
single living cell with spatial, temporal, and qualitative
dosage control, which potentially applicable for medical
diagnostics and therapeutic studies.