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Tissue Science 2019
June 17-18, 2019
London, UK
Advances in Tissue
Engineering and
Biomaterials Science
13
th
Edition of International Conference on
Journal of Biomedical Sciences
ISSN: 2254-609X
Page 34
Y. Khalifa et al., J Biomedical Sci 2019, Volume 08
The effect of nanoparticle labeled bone marrow-derived
mesenchymal stem cells as a therapeutic strategy for
experimentally induced liver fibrosis in albino rats
Y. Khalifa, G. Mourad,W. Stephanos, S. Omar
and
R. Mehanna
Alexandria University, Egypt
Objectives:
This study aims at exploring the therapeutic
efficacy of superparamagnetic iron oxide nanoparticles
(SPIO) labeled bone marrow derived mesenchymal stem
cells (BM-MSCs) on carbon tetrachloride (CCl4) induced
liver fibrosis in adult female albino rats.
Material andmethods:
MSCswereobtained from10male
Sprague Dawley rats and 50 female rats were assigned
into 2 groups; control group (CG) and experimental group
(EG). EG was subdivided into three subgroups. Induction
group by intraperitoneal injection of CCl4 for 8 weeks,
MSCs treated + CCl4 group (MSCs+CCl4G) received
SPIO- BM-MSCs simultaneouslywithCCl4 administration
to assess the effect of SPIO-BM-MSCs on the prevention
of progression of liver fibrosis with the persistence
of the cause. MSCs treated group (MSCsG), received
SPIO-BM-MSCs after withdrawal of CCl4. The rats were
sacrificed after 8 weeks and assessed by histological
examination, liver function tests, transforming growth
factor-beta (TGF-ß1) immunofluorescence staining,
PCR for quantification of the gene expression levels of
matrix metalloproteinase-1(MMP-1) and tissue inhibitor
of metalloproteinase-1 (TIMP-1).
Results:
SPIO labeled MSCs were engrafted in the
fibrotic liver and MSCs improved liver functions,
enhanced the gene expression of MMP-1, whereasTIMP-
1 gene expression was depressed. Histological and
morphometric studies confirmed these results.
Conclusion:
BM-MSCs prove to be a promising therapy
for liver fibrosis.
Biography
Khalifa is an assistant lecturer of Histology and Cell Biology
(23/9/2014- now), Department of Histology and Cell Biolo-
gy, Faculty of Medicine, Alexandria University, Egypt. She is a
member of the stem cell research group, Faculty of Medicine,
AlexandriaUniversity, Egypt. (Oct 2015- present). In September
2014, she was registered for Doctorate Degree (MD) in Histolo-
gy & Cell biology, Faculty of Medicine, University of Alexandria.
Yasmine.hossam84@gmail.com