tissue-science-2019-posters-abstracts

2 / 14

Tissue Science 2019
June 17-18, 2019
London, UK
Advances in Tissue
Engineering and
Biomaterials Science
13th Edition of International Conference on
Journal of Biomedical Sciences
ISSN: 2254-609X Page 30
Asma Alanazi et al., J Biomedical Sci 2019, Volume 08
Comparative adhesive and migratory properties of
mesenchymal stem cells from different tissues
Asma Alanazi1,3, Hafsa Munir1, Mohammed Alassiri1,3, Lewis S.C. Ward, Helen
M. McGettrick 2 and Gerard B. Nash1
1King Saud bin Abdulaziz University for Health Sciences, Saudi Arabia
2University of Birmingham, UK
3Abdulaziz University for Health Sciences, KSA
Mesenchymal stem cells (MSC) are used in therapy,
often by injection into the blood. We aimed to
compare the adhesive and migratory properties of MSC
from umbilical cords (UCMSC), bone marrow (BMMSC)
or trabecular bone (TBMSC), which might influence
delivery to injured tissue.MSC were perfused through
glass capillaries coated with matrix proteins, collagen
or fibronectin, or albumin. Adherent cells were counted
microscopically and their spreading analysed over
time. MSC migration through 8µm pore filters coated
with the same proteins was analysed. The number of
MSC adhering to collagen was greater than fibronectin,
decreased as wall shear rate increased from 17 to 70s-
1, and was in the order UCMSC>BMMSC>TBMSC.
Conversely, spreading was more effective on fibronectin
and was in the order BMMSC>TBMSCUCMSC. Migration
was promoted by coating the lower surface of filters
with either matrix protein, with UCMSC migrating more
efficiently than BMMSC. MSC show origin-dependent
variations in their efficiency of capture from flow and
subsequent spreading or ability to migrate on matrix
proteins. UCMSC showed most efficient capture from
flow, which was followed by less spreading, but more
rapid migration. These responses might be associated
with more effective delivery from the circulation into
damaged tissue.
Biography
Asma Alanazi, assistant professor of Physiology in College of
Medicine at King Saud bin Abdulaziz University for Health Sci-
ences, Riyadh, KSA.