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E u r o S c i C o n J o i n t E v e n t s o n
Plant Science, Tissue Engineering
and Parasitology
December 03-04 , 2018
Amsterdam, Nether l ands
International Journal of Applied Science - Research and Review
ISSN: 2394-9988
Plant Science | Tissue Engineering | Parasitology 2018
T
o provide a bioactive and biocompatible environment for new bone formation, several materials have been used to mimic
the bone-forming components. In this context, we examined the feasibility of the natural polyphenol oleuropein as a cross-
linking agent for fabricating a polymerizable biomaterial for potential applications in bone tissue engineering. Oleuropein is a
natural-occurring iridoid glycoside especially found in the leaves and fruits of the olive tree (
Olea europaea
). It is well known for
its antibacterial, antifungal, antiviral, antioxidant and anti-inflammatory activities. More importantly, it has also been shown that
oleuropein is able to induce bone formation and inhibits bone resorption as well as bone tissue demineralization. Interestingly,
the conversion of oleuropein glucoside to its aglycone by the enzyme β-glucosidase exhibits interesting cross-linking properties
which make it a suitable cross-linking agent for biomedical applications. Here, the autologous patients’ derived-plasma and
aglycone oleuropein were used to fabricate a novel biomaterial. We invented a novel formulation in which autologous patients
derived plasma is used as protein substrate that is able to polymerize when is mixed with the aglycone oleuropein. By using L929
mouse fibroblasts and dental pulp stem cells (DPSCs), we demonstrated that this biopolymer is nontoxic and biocompatible.
Importantly, plasma-derived biomaterial contains biochemical signals necessary for tissue repair and regeneration and can be
easily generated from patient’s autologous blood without the potential risk of foreign body reaction or infection. Although this
biomaterial shows interesting potentiality, several investigations are required to elucidate the potential of this novel biomaterial
for bone tissue engineering applications.
francesco.paduano@tecnologicasrl.comA novel natural and patient-derived biomaterial
fabricated using the polyphenol oleuropein
and the autologous blood-derived plasma for
potential applications in bone tissue engineering
Francesco Paduano, Massimo Marrelli, Bruna Codispoti and
Marco Tatullo
Tecnologica Research Institute and Marrelli Health, Italy
Int J Appl Sci Res Rev 2018, Volume: 5
DOI: 10.21767/2394-9988-C2-006