22 / 33
Page 64
E u r o S c i C o n J o i n t E v e n t s o n
Plant Science, Tissue Engineering
and Parasitology
December 03-04 , 2018
Amsterdam, Nether l ands
International Journal of Applied Science - Research and Review
ISSN: 2394-9988
Plant Science | Tissue Engineering | Parasitology 2018
A
spergillus flavus
is the main producer of carcinogenic aflatoxins in agricultural commodities such as maize. This fungus
produces aflatoxin B1 (AFB
1
) and aflatoxin B
2
(AFB
2
), being the most relevant in crops and this can result in economic
losses. The aim of this study was to investigate four strains of
A. flavus
field for the production of aflatoxin B
1
and aflatoxin B
2
.
The strains: 3909, 3911, 3951 and 3955 are isolated from Lydenburg in Mpumalanga were morphologically identified at ARC-
Plant Protection Research Institute and were further characterised by Polymerase Chain Reaction (PCR) and Sanger sequencing
of the internal transcriber subunit regions: ITS-5.8-ITS2. The strains were analysed for the presence of genes encoding AFB
1
,
targeting both regulatory (
aflR, aflS
) and structural genes (
aflD, aflM, aflO, aflP and aflQ
). To determine the actual production of
aflatoxin B
1
and B
2
of the four strains, a reverse high performance liquid chromatographic (HPLC) instrument was used. All the
four strains amplified 600bp of ITS-5.8-ITS2 rDNA region. Similarly, all of seven genes for aflotoxin B1 were detected in four
strains with expected band sizes. Aflatoxin production was present in strain 3911 and 3955 for AFB
1
and AFB
2
and in strain 3951
only AFB
1
while strain 3909 revealed negative aflatoxin (AFB
1
and AFB
2
) production. The results may contribute to development
of reliable molecular techniques for detection of aflatoxigenicity as well as illustrating the complexity of local fungal communities
associated with maize.
athini.07@gmail.comCharacterisation of
Aspergillus flavus
isolated
from maize
Athini Ntloko
1
, G Kwinda2, O Abafe
1
, A Jacobs
2
, N N Ludidi
2
and
N Gcebe
2
1
Plant Biotechnology Research Group-University of Western Cape, South Africa
2
Agricultural Research Council, South Africa
Int J Appl Sci Res Rev 2018, Volume: 5
DOI: 10.21767/2394-9988-C2-006