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E u r o S c i C o n J o i n t E v e n t s o n
Plant Science, Tissue Engineering
and Parasitology
December 03-04 , 2018
Amsterdam, Nether l ands
International Journal of Applied Science - Research and Review
ISSN: 2394-9988
Plant Science | Tissue Engineering | Parasitology 2018
T
he ability of skeletal muscle to self-repair after a traumatic injury, tumour ablation or muscular disease is slow; limited, and
declines steeply with age. Tissue engineering of functional skeletal muscle using 3D bioprinting technology is promising for
creating tissue constructs that repair and promote regeneration of damaged tissue. Hydrogel scaffolds used as biomaterials
for skeletal muscle tissue engineering can provide chemical, physical and mechanical cues to the cells in three dimensions
thus promoting regeneration. Herein, we have developed two synthetically designed novel tetramer peptide biomaterials. These
peptides are self-assembling into a nanofibrous 3D network, entrapping 99.9% water and mimicking the native collagen of an
extracellular matrix. Different biocompatibility assays including MTT, 3D cell viability assay, cytotoxicity assay and live-dead assay
confirmed the biocompatibility of these peptide hydrogels for mouse myoblast cells (C2C12). Immunofluorescence analysis of
cell-laden hydrogels revealed that the proliferation of C2C12 cells was well-aligned in the peptide hydrogels compared to the
alginate gelatin control. These results indicate that these peptide hydrogels are suitable for skeletal muscle tissue engineering.
Finally, we tested the printability of the peptide bioinks using a commercially available 3D bioprinter. The ability to print these
hydrogels will enable future development of 3D bioprinted scaffolds containing skeletal muscle myoblasts for tissue engineering
applications.
Wafaa.arab@kaust.edu.saNovel ultrashort self-assembling peptide bioinks
for 3D culture of muscle myoblast cells
Wafaa Arab1, Sakandar Rauf
1
, Ohoud Al-Harbi
2
and Charlotte A
E Hauser
1
King Abdullah University of Science and Technology, Saudi Arabia
Int J Appl Sci Res Rev 2018, Volume: 5
DOI: 10.21767/2394-9988-C2-006