plant-science-tissue-engineering-parasitology-2018-posters

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E u r o S c i C o n J o i n t E v e n t s o n

Plant Science, Tissue Engineering

and Parasitology

December 03-04 , 2018

Amsterdam, Nether l ands

International Journal of Applied Science - Research and Review

ISSN: 2394-9988

Plant Science | Tissue Engineering | Parasitology 2018

aize has become a significant crop all over the world owing to its potential for fulfilling food demand of people. Present study

was carried out to evaluate genetic variations among maize genotypes through SSR markers. For genetic diversity analysis

15 genotypes of maize collected from different research institutes of Punjab Pakistan. Several growth and quality parameters

were also examined such as fresh root weight, fresh shoot weight, dry shoot weight, dry root weight, crude protein, crude fat,

ash content, acid detergent fiber and neutral detergent fiber. On the basis of reproducibility and high PIC value, 22 SSR primers

were selected for DNA finger printing of all collected genotypes. Binary method was used for band scoring. The polymorphism

information content (PIC) value of primers was calculated to estimate extent of genetic differences. It was calculated that

polymorphism information content ranged from 0.1 to 0.8. Only three markers umc1122, umc1568 and umc1988 provided

highly polymorphic values which were 0.8, 0.5 and 0.6 respectively. Unweighted Paired Group Method using Arithmetic Averages

(UPGMA) cluster analysis has grouped the genotypes into three major clusters. Cluster analysis revealed that maximum genetic

distance was found 3.16 between Sgd 2002 and Golden, while minimum genetic distance was observed 1.414 between Fsd

maize and Neelam. The identified clusters may find useful to plan crossing program in maize breeding. This study concluded

SSR as a good source for genetic characterization. It was cleared from phlylogenetic analysis that genetic diversity among above

mentioned genotypes was limited since all these genotypes were collected from nearby areas in province of Punjab and there

were no exotic genotype. On the other hand, data recorded for growth and quality parameters subjected to statistical analysis and

results showed all maize genotypes had considerable genetic differences for all growth and quality traits and maximum genetic

variability was examined for fresh shoot weight, fresh root weight, dry shoot weight, dry root weight, crude protein, crude fat, acid

detergent fiber (ADF), neutral detergent fiber (NDF) and ash content in Sgd 2002 (13.5g), Agaiti 72 (18g), Fsd maize 2018 (6.8g),

Agaiti 72 (8.7g), EY 1098 (5.1g), YHM (1.9g), Pearl maize (30g), Agaiti 2002 (49g) and MS 2010 (30g) respectively. These findings

of genetic diversity analysis among maize genotypes with the help of SSR markers as well as on basis of growth and quality traits

may be consider for further evaluation and selection as parents in breeding programmes.

Evaluation of genetic diversity among maize

genotypes using SSR markers

Ayesha Babar, H M N Cheema and Z Babar

University of Agriculture Faisalabad, Pakistan

Int J Appl Sci Res Rev 2018, Volume: 5

DOI: 10.21767/2394-9988-C2-006