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E u r o S c i C o n J o i n t E v e n t s o n
Plant Science, Tissue Engineering
and Parasitology
December 03-04 , 2018
Amsterdam, Nether l ands
International Journal of Applied Science - Research and Review
ISSN: 2394-9988
Plant Science | Tissue Engineering | Parasitology 2018
L
ernaea
, a copepod parasite commonly known as (Anchor worm) contamination is a major sickness issue experienced in fish
culture in the Indian subcontinent. Different developmental stages in parasites and numerous associated impacts of full scale
and small scale, environment impact the parasitic fauna of fish. The present study reveals the external morphology and control
the highly infested copepod parasite
Lernaea cyprinacea
which have contributed disease problems to the aquaculture industry
in Pakistan. The external morphometric parameters i.e. full body length, length of cephalothorax, length of thorax region, length
of abdomen, length of dorsal and ventral head region, length of dorsal and ventral anchors, length of dorsal anterior and dorsal
posterior anchor, length of 1st and 2nd maxillae, length mandible, length of dorsal and ventral antennule were measured and
statistically analysed for mean, standard deviation, standard error, coefficient of variability, confidence interval (95%) and analysis
of variance. To determine anti-copepods potential of ethanol extracts of leaves of
Carica pappaya, Euclyptus camaldulensis and
Grevillea robusta
, 10 infected fish with three average copepod parasites were placed in each glass aquarium treated with different
concentrations (10 ppm, 25 ppm, 50 ppm). The survival percentage of
L.cyprinacea
copepod was observed after 6 hrs, 12 hrs,
24 hrs, 36 hrs, 48 hrs, 60 hrs and 72 hrs. The results were analysed by using one way Anova and Tukey test. Among all extracts
bioassays, the maximum activity was observed in extracts of
Grevillea robusta, Carica pappaya, Euclyptus camaldulensis
. To
estimate the toxicity level of each plant, extract was calculated by determining LC
50
and LC
90
. To estimate the protein (mg/g),
carbohydrate (mg/g) and lipid mg/dl (cholesterol) level in effected copepods were estimated by Lowry’s method, phenol sulphuric
acid method by biochemistry analyser. Protein, carbohydrate and lipid contents of copepod parasite were decreased as compared
to control.
rehman.azam@outlook.comMorphological characterization and plant based
control of Lernaea cyprinacea copepod fish
parasite
Abdur Rehman Azam
1
, Naveeda Akhtar Qureshi
1
, Tooba Latif
2
and Shaista Bibi
3
1
Quaid I Azam University Islamabad, Pakistan
2
University of Veterinary and Animal Sciences, Pakistan
3
Pir Mehar Ali Shah Arid Agriculture University, Pakistan
Int J Appl Sci Res Rev 2018, Volume: 5
DOI: 10.21767/2394-9988-C2-006