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E u r o S c i C o n J o i n t E v e n t s o n
Plant Science, Tissue Engineering
and Parasitology
December 03-04 , 2018
Amsterdam, Nether l ands
International Journal of Applied Science - Research and Review
ISSN: 2394-9988
Plant Science | Tissue Engineering | Parasitology 2018
H
uman toxocariasis is an important worldwide soil-transmitted zoonotic disease. Neurotoxocariasis is a serious condition that
is linked to reduced cognitive function, behavioural alterations and neurodegenerative diseases. Unfortunately, the available
drugs for treatment of toxocariasis are with variable results. Mesenchymal stem cells (MSCs) have been used in animal models
and clinical trials of tissue injuries and it gave promising therapeutic results. Therefore, this study was designed using 40
T. canis
-
infected albino mice (1000 eggs/mouse, orally) and an additional control group (GI) of 10 healthy mice. The infected mice were
divided into four groups (n=10). GII was the infected non-treated group (infected control), GIII was treated with albendazole at
a dose of 100 mg/kg/d once orally for 5 successive days, GIV was treated with bone marrow derived MSCs at a dose of 3x106
MSCs in 0.1 mL of PBS via the tail vein, and GV was treated with albendazole + MSCs. Treatment was commenced 6 weeks p.i.
and the experiment was terminated four weeks after administration of the last doses of the tested drugs. The brain tissue of
each mouse was subjected for histopathological, immunehistochemical studies (caspase-3, TGF-β), detection or
T. canis
DNA by
real-time PCR and gene expression the biomarkers of brain damage (S100B, GFAP) by RT-PCR. Moreover, homing of iron oxide-
labelled MSCs in brain tissues was assessed by Prussian blue stain. The brain tissues of GII showed numerous
T. canis
larvae,
significant congestion, thickening of arterioles, inflammatory infiltrate and gliosis associated with marked immunohistochemical
expression of TGF-β and caspase-3 as well as marked S100B and GFAP gene expression. Significant improvements of the
previous parameters and
T. canis
DNA were recorded in all the treated groups. However, the best results were obtained with
combined albendazole + MSCs therapy. Thus, MSCs could be considered in the treatment of chronic neurotoxocariasis.
dr.fatma.shaalan@gmail.comMesenchymal stem cell as a cure for brain damage
induced by chronic Toxocara canis infection in an
experimental mouse model
Fatma Hamed Shalan
1
, Gehan Salah Sadek
1
, Ayman Abd El-Moen-
em Elbadry
2
, Amera Fathy Afifi
1
, Engy Victor Nassief Beshay
1
, Sa-
mar Ahmed El-Refai
1
, Hala Gabr Metwaly
2
, Eman Ahmady
1
, Eman
Abd El-Fattah Badr
1
, Dalia Refaat Al-Sharaky
1
1
Menoufia University, Egypt
2
Cairo University, Egypt
Int J Appl Sci Res Rev 2018, Volume: 5
DOI: 10.21767/2394-9988-C2-006