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E u r o S c i C o n J o i n t E v e n t s o n
Plant Science, Tissue Engineering
and Parasitology
December 03-04 , 2018
Amsterdam, Nether l ands
International Journal of Applied Science - Research and Review
ISSN: 2394-9988
Plant Science | Tissue Engineering | Parasitology 2018
U
rban malaria is becoming a major public health problem in Cameroon. In the frame of a larval control study due to be
implemented in the city of Yaoundé, we conducted baseline surveys to assess malaria transmission dynamic in this city.
Adult mosquitoes were collected indoor and outdoor using light traps of Center of Diseases Control and Human Landing Catches
from Mar’ 2017 to Mar’ 2018 in 30 districts of the city. Mosquitoes were sorted by genus and identify up to the species level
using polymerase chain reaction (PCR). TaqMan
Plasmodium
infection and ELISA CSP analysis were used to determine mosquito
infection status. The detection of Knock down resistance (kdr) mutation was carried out on infected and non-infected
An. gambiae
s.l. using DNA-based molecular technique. A total of 218,991 mosquitoes were collected. The main malaria vectors were
An.
gambiae
s.l. (n=6154) and An. funestus s.l. (n=229). PCR results (n=1622) revealed that 92.29%
An. coluzzii
and 7.71%
An.
gambiae
s.s. constituted
An. gambiae
s.l. Amongst the 186 An. funestus s.l. analysed by PCR, 93.51% belonged to An. funestus
s.s. and 2.16% to
An. leesoni
. The average biting rate of
An. gambiae
s.l. was higher outdoor than indoor (P=0.013). Seasonal
variation in mosquito abundance and biting rate was recorded as well as variation according to districts. Furthermore, the
P.
falciparum
infection rate was 2.15% and the annual entomological infection rate was estimated at 80.49 infective bites. There
was no significant difference between the resistance allele frequencies of infected and non-infected females (P=0.49). Thus, the
susceptibility of
An. gambiae
s.l. to
P. falciparum
was not affect by the presence of the resistance alleles at the kdr locus. The
results provide evidence of increasing outdoor malaria risk in the city of Yaoundé and call for more actions to improve control
strategies in this city.
Lucie.doumbe@yahoo.frHigh malaria transmission sustained by Anopheles
gambiae s.l. and status of kdr resistance mutation
of Plasmodium falciparum infected specimens in the
city of Yaoundé Cameroon
Doumbe-Belisse P
1, 2
, Ngadjeu C S
1, 2
, Talipouo A
1, 2
, Djamou-
ko-Djonkam L
1, 3
, Kopya E
1, 2
, Bamou R
1,3
, Sonhafouo-Chiana N
1,4
,
Wondji C
5
, Awono-Ambene P
1
, Njiokou F
2
and Antonio- Nkondjio C
1,5
1
Laboratoire de Recherche sur le Paludisme-OCEAC, Cameroon
2
University of Yaoundé, Cameroon
3
University of Dschang, Cameroon
4
University of Buea, Cameroon
5
Vector Group Liverpool School of Tropical Medicine, UK
Int J Appl Sci Res Rev 2018, Volume: 5
DOI: 10.21767/2394-9988-C2-006