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allied
academies
Ann Biol Sci, 2017
ISSN: 2348-1927
August 23-24, 2017 | Toronto, Canada
Annual Conference on
MICROBIAL PATHOGENESIS, INFECTIOUS DISEASE,
ANTIMICROBIALS AND DRUG RESISTANCE
Introduction:
The non-toxin production variant
C. difficile
A
-
/
B
-
/CDT
-
are prevalent in clinical samples. But the reason for
their high prevalence of these strains in the clinical diarrhea
specimens has not yet been performed.
Materials & Methods:
Minimum inhibitory concentration
bacteria were performed by micro- dilution technique. About
~10
6
bacteria from 18-hour culture were inoculated to pre-
reducedmedia containing ½×MIC of each antibiotic. After 24,
48 and 96 hours, 1/mL of culture was excluded and heated
to killing vegetative forms and pre-activated the spores. The
100 of appropriate dilution are cultured on Columbia blood
agar in the form of triplicates. After 72 hours the number of
spore were counted based on the colony forming unit.
Results:
The results showed that non-toxigenic isolates and
historically strain of
C. difficile
(ATCC 9689) and the clinically
isolates A
+
/B
+
/CDT
-
produced spore in free antibiotic and
½×MIC media. The spore production non-toxigenic isolates
in free antibiotic media was like toxigenic (clinically and
ATCC 9689 strain). The VAN, CLI and CAZ inhibited spore
production in toxigenic as the same as non-toxigenic isolates
(A
-
/B
-
/CDT
-
) of
C. difficile
in the similar manner.
Discussion:
Since non-toxigenic isolates are common in
the clinical samples. Our research showed these isolates
capable to produce spore in absence and the presence of
antibiotic in similar manner to toxigenic strain. In total, they
have lost toxin production ability but they kept the power
sporulation and survival in the hospitalized patients who
receive antibiotics.
Speaker Biography
Mohammad Moradi completed his PhD in Medical Microbiology, University of
Manchester. He is currently working as a Assistance Professor of Medical Microbiology
in the Department of Medical Microbiology Medical School, Kerman University of
Medical Sciences, Kerman, Iran. He has published numerous research papers and
articles in reputed journals and has various other achievements in Molecular diagnosis
and anti-microbial resistance patterns. He has extended his valuable service towards
the scientific community with his extensive research work.
e:
m_moradie@yahoo.co.ukThe reason for the prevalence of non-toxigenic isolates of
Clostridium difficile
in the clinical samples
Mohammad Moradi, Ebrahim Rezazadeh Zarandi, Shahla Mansouri, Nouzar Nakhaee
and
Farhad Sarafzadeh
Kerman University of Medical Sciences, Iran
Mohammad Moradi et al., Arch Clin Microbiol, 8:5
DOI: 10.4172/1989-8436-C1-003