15 / 38
Page 72
allied
academies
Ann Biol Sci, 2017
ISSN: 2348-1927
August 23-24, 2017 | Toronto, Canada
Annual Conference on
MICROBIAL PATHOGENESIS, INFECTIOUS DISEASE,
ANTIMICROBIALS AND DRUG RESISTANCE
M
any lactic acid bacteria (LAB) were isolated from
‘Shermout’, a popular Sudanese fermented beef
product intended for long storage. An isolate that
demonstrated significant antibacterial activity was identified
as Lactobacillus plantarum
PM4 based on phenotypic,
physiological
and
biochemical
characteristics
and
carbohydrate utilization patterns. The inhibitory activity of
the partially purified bacteriocin was completely arrested by
the proteolytic enzymes proteinase-k and pepsin but not by
α-amylase, asserting its proteinaceous nature. The activity
was not due to H2O2 as similar inhibition was obtained
by cell-free supernatant (CFS) produced under anaerobic
conditions. The bacteriocin showed a molecular weight in
the range of 3-5 kDa and had a bactericidal mode of action.
No significant reduction in activity was observed on heating
at 60°C for 60 min, but activity was lost on heating at 100°C
or autoclaving. Highest inhibitory activity was at pH 5.5 and
there was appreciable reduction in activity at pH 3, 7 or 9.
There was no drop-in activity at -80 or -20°C up to four weeks
of storage. However, at 4 and 35°C a gradual decline in activity
was observed.
L. plantarum
PM4 exhibited bactericidal
activity against
Staphylococcus aureus, Bacillus subtilis,
Enterococcus faecalis, Escherichia coli
ATCC25922,
Klebsiella
pneumoniae and Proteus vulgaris
. Bacteriocin production
generally coincided with the phase of maximum growth and
the best combination for maximum production of inhibitory
activity was at pH 5.5 for 48 hours, further incubation at 25,
30 or 37°C.
L. plantarum
PM4 showed promise as a starter
culture in the fermentation of preserved meat products.
e:
idress174@gmail.comCharacterization and optimization of bacteriocin from
Lactobacillus
plantarum
isolated from
fermented beef
Idress Hamad Attitalla
Omar Al-Mukhtar University, Libya
Arch Clin Microbiol, 8:5
DOI: 10.4172/1989-8436-C1-003