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allied
academies
Ann Biol Sci, 2017
ISSN: 2348-1927
August 23-24, 2017 | Toronto, Canada
Annual Conference on
MICROBIAL PATHOGENESIS, INFECTIOUS DISEASE,
ANTIMICROBIALS AND DRUG RESISTANCE
Arch Clin Microbiol, 8:5
DOI: 10.4172/1989-8436-C1-003
Background:
Glucose
6-phosphate
dehydrogenase
deficiency (G6PDd) is an X-linked hereditary genetic defect,
affecting an estimated 400 million people worldwide. Severe
clinical manifestations associated with G6PDd (e.g., chronic
hemolytic anemia) depends on the type of G6PD molecular
variants and exposure to hemolytic triggers (e.g., antimalarial
like Primaquine). However, a scarce study on G6PDd renders
the use of Primaquine for effective therapeutic treatment of
malaria.
Objectives:
To determine the availability and characterization
of selected molecular variants of G6PDd specific genes
among selected populations in malaria endemic area of
Amhara region, Ethiopia.
Methods:
Using a cross sectional study design, a total of
156 dried blood samples were randomly selected from 360
stored samples of national malaria indicator survey of 2011
starting from July 30/2014 to January 30/2015. Polymerase
chain reaction and restricted fragment length polymorphism
technique was applied to characterize G6PDd variants as
G6PD*A, G6PD*A- and/or G6PD*Mediterranean. Binary
logistic regression was applied to see association (P<0.05 is
significant) among different parameters.
Result:
Of 156 studied dried blood spot samples, 10 (6.4%)
had G6PD genotype available. G6PD*A (100%) was the
only genotype characterized, while neither G6PD*A- nor
G6PD*Mediterranean genotypes were detected. There was
no statistical significant difference between G6PDd and
other socio demographic and risk related variables (P>0.05).
Conclusion:
G6PD*A variant was the only G6PDd genotype
detected in this study. G6PD*A variant has almost (90%) the
same enzymatic activities with the wild type. Therefore, this
result supports the safe use of primaquine, especially the
single low dose for transmission interruption of Plasmodium
falciparum gametocyte and radical cure of
Plasmodium
vivax
, as a part of malaria elimination toolkit, among selected
populations in malaria endemic areas of Amhara region.
e
:
y_mamuye@yahoo.comMolecular characterization of glucose-6-phosphate dehydrogenase deficiency specific variants in
Amhara region, Ethiopia
Yeshwondm Mamuye
1
and Getachew Abebe
2
1
St.Paul’s Hospital Millennium Medical College, Ethiopia
2
Asela University School of Laboratory, Ethiopia