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Page 78
allied
academies
Ann Biol Sci, 2017
ISSN: 2348-1927
August 23-24, 2017 | Toronto, Canada
Annual Conference on
MICROBIAL PATHOGENESIS, INFECTIOUS DISEASE,
ANTIMICROBIALS AND DRUG RESISTANCE
E
nterobacteriaceae
are Gram-negative rods causing
serious infections in intensive care units (ICUs) of
hospitals. These organisms are showing resistance to
several classes of antimicrobials and resistance genes are
spreading by acquired plasmids in bacterial population.
Resistance to carbapenem group of antimicrobials is an
emerging problem for clinicians and surgeons. Isolation,
identification and molecular characterization of carbapenem
resistant
Enterobacteriaceae
(CRE) from patients admitted in
intensive care units of tertiary care hospital. This research
is a prospective, non-randomized, descriptive study. In 9
months, 83 isolates of CRE from ICU samples were processed
in Department of Pathology, SZABMU, PIMS Pakistan. Out of
83 CRE samples, 26.5% were from urine, 26.5% were from
endotracheal tube tip, 14% were from blood, 13% were
from pus, 13% were from tracheal secretions, 4% were from
fluids and 3% were from catheter tip. 75% were
Klebsiella
pnemoniae
, 17% were E. coli, 2.25% were
Klebsiella
specie,
2.25% were Enterobacter agglomerans, 2.25% were
Enterobacter cloacae
and 1.25% was
Klebsiella oxytoca
. CRE
are100%resistant to imipenem,meropenemandertapenem.
Tigecycline is the only parental drug which is found effective
against CRE isolates. 14.5% of CRE isolates was sensitive to
amikacin. MIC of imipenem showed 100% resistance for CRE
isolates. NDM gene was present in 28 (56%) samples. VIM
gene, KPC gene, IMP gene were not detected. NDM positive
isolates were 48%
Klebsiella pneumoniae
.
e:
azkafatima@gmail.comMolecular characterization of Carbapenem resistant
Enterobacteriaceae
from intensive care units of
a tertiary care hospital of Islamabad
Azka Fatima
Shaheed Zulfiqar Ali Bhutto Medical University, USA
Arch Clin Microbiol, 8:5
DOI: 10.4172/1989-8436-C1-003