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Ann Biol Sci, 2017
ISSN: 2348-1927
August 23-24, 2017 | Toronto, Canada
Annual Conference on
MICROBIAL PATHOGENESIS, INFECTIOUS DISEASE,
ANTIMICROBIALS AND DRUG RESISTANCE
P
seudomonas aeruginosa
(Pa) and
Candida albicans
(Ca) are major bacterial and fungal pathogens in
immunocompromised hosts, and notably in the airways of
cystic fibrosis patients. Bacteriophages of Pa physically alter
biofilms, and have been recently shown to inhibit biofilms of
Aspergillus fumigatus
. To understand the range of this viral-
fungal interaction, we studied Pa phages Pf4 and Pf1, and
their interaction with Ca biofilm formation and preformed
Ca biofilm. Both forms of Ca biofilm development, as well as
planktonic Ca growth, were inhibited by both phages. The
inhibition of biofilm was reversed by the addition of iron,
suggesting the mechanism of phage action on Ca is denial
of iron. Birefringence studies on added phage showed an
ordered structure of binding to Ca. Electron microscopic
observations indicated phage aggregation in biofilm
extracellular matrix. Phage-fungal interactions may be a
feature with several pathogens in the fungal kingdom.
Speaker Biography
Hasan Nazik has completed his undergraduate degree at Istanbul University, School
of Medicine-Istanbul/Turkey in 2001, and his Microbiology Residency education
at the same University in 2005. He is a Visiting Scholar at California Institute for
Medical Research/Stanford University for three years. He has published more than 40
papers in scholarly journals and has been continuing the research on bacterial-fungal
interactions.
e:
hasannazik01@gmail.comPseudomonas
phage inhibition of
Candida albicans
Hasan Nazik
1, 2
, Lydia-Marie Joubert
2
, Patrick R Secor
3
, Jolien Sweere
2
, Paul L Bollyky
2
, Gabriele Sass
1
, Lynette Cegelski
2
and
David A Stevens
1, 2
1
California Institute for Medical Research, USA
2
Stanford University Medical School, USA
3
University of Montana, USA
Hasan Nazik et al., Arch Clin Microbiol, 8:5
DOI: 10.4172/1989-8436-C1-003