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allied
academies
Ann Biol Sci, 2017
ISSN: 2348-1927
August 23-24, 2017 | Toronto, Canada
Annual Conference on
MICROBIAL PATHOGENESIS, INFECTIOUS DISEASE,
ANTIMICROBIALS AND DRUG RESISTANCE
Background & Objectives:
Cutaneous leishmaniasis (CL) is
one of the most common parasitic diseases. It is one of the
major public health concerns in developing countries and
throughout the world. Pentavalent antimonial compounds
like pentostam and glucantime has been used to treat
CL for the last 50 years. The use of these compounds has
some limitations such as long duration of treatment, high
expenses of drugs, and methods of drug use which are
intradermal and intramuscular injection. Beside these, lack
of response to the treatment in 10-15% of cases and toxic
effects on heart, liver, and kidney are other possible side
effects [4-6]. Hence, the objective of the present survey was
to state the antileishmanial activity of two herbal medicine
(
Artemisia dracunculus
and
Heracleum persicum
) extracts
were evaluated against
Leishmania major
and
Leishmania
infantum
using colorimetric MTT (2-(4,5-dimethyl-2-
thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide) assay and
compared to the Glucantime as a reference.
Methods:
The leaf extracts of selected plants were
obtained by maceration. The in vitro assays were carried
out on
Leishmania major
and
Leishmania infantum
using
colorimetric MTT assay in comparison with Glucantime.
The concentration-response curves tested extracts and
glucantime solutions were designed and IC50 values were
located.
Results and Conclusions:
Anti-leishmania effects of
Artemisia
dracunculus
and
Heracleum persicum
on
L. major
and
L.
infantum
promastigote were revealed with 50% inhibitory
concentration (IC50) values of 50.97 and 49.57 mg ml
-1
for
Artemisia dracunculus
, 29.3 and 14.7 mg ml-1 for
Heracleum
persicum
. In comparison with the standard drug, glucantime
had IC50 value of 40.2 mg ml
-1
for
L.major
and 18.5 mg ml
-1
for L. infantum promastigote after 72 hours incubation,
respectively.
Conclusions:
These results revealed that compounds from
Satureja khuzestanica
and
Heracleum persicum
have anti-
leishmania properties that necessary to survey the effects
of these extracts on leishmania genus in animal models in
future.
Speaker Biography
Batool Sadeghi-Nejad is working at Abadan School of Medical Sciences, Iran
e:
atsad4@yahoo.comIn vitro
anti-leishmanial activity of
Artemisia dracunculus
and
Heracleum persicum
extracts in
comparison with glucantime
Batool Sadeghi-Nejad
1
, Shahram Khademvatan
2
, Alborz Eskandari
3
and
Sedigheh Yusef Naanaie
4
1
Abadan School of Medical Sciences, Iran
2
Urmia University of Medical Sciences, Iran
3
Ahvaz Jundishapur University of Medical Sciences, Iran
4
The Agricultural and Natural of Resources center, Iran
Batool Sadeghi-Nejad et al., Arch Clin Microbiol, 8:5
DOI: 10.4172/1989-8436-C1-003