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Ann Biol Sci, 2017
ISSN: 2348-1927
August 23-24, 2017 | Toronto, Canada
Annual Conference on
MICROBIAL PATHOGENESIS, INFECTIOUS DISEASE,
ANTIMICROBIALS AND DRUG RESISTANCE
S
treptococcus mutans
is a leading cariogenic pathogen
of dental caries worldwide. Clinically, eliminating
S.
mutans
from dental biofilms using antibiotics is not practical,
because these agents indiscriminately kill other members
of the resident microflora, leading to ecological disruption
and other negative clinical consequences. To develop
target-specific antimicrobials, we evaluated several fusion
peptides and identified a new peptide HP30 that showed a
high selectivity for targeted killing of
S. mutans
. In the dual-
species cultures, 80% of
S. mutans
cells were killed, but only
20% of
S. sanguinis
were killed following exposure to HP30
(5.0
μ
M) for 15 min. Similarly, 80% of
S. mutans
cells were
killed but only 5% of
Actinomyces naeuslundii
were killed
following the same exposure. The peptide-guided killing was
also confirmed in the dual-species biofilms and the killing
increased with increasing concentrations of HP30. However,
a combination of low concentrations of HP30 with EDTA
well maintained the killing activity against
S. mutans
in the
biofilms. A
S. mutans
mutant lacking the ComD receptor only
showed 20% of killing, while a ComD overexpression strain
showed 90% of killing, suggesting that HP30 predominantly
binds to the ComD receptor before triggering the selective
killing. New peptide HP30 displays a high selectivity for
targeted killing of
S. mutans
due to an improved binding of
the peptide to the ComD receptor.
Speaker Biography
Xiao-Lin Tian has received her MD from Shanghai Medical University. Since 1993, she
worked as a Research Technician in Novopharm Biotech Inc. in Winnepeg for six years.
She then worked in the Mount Sinai Hospital Lunenfeld Research Institute, Toronto,
for another six years. Since 2006, she has been working as a Researcher at Dalhousie
University, with expertise in Moleculr Biology, Bacterial Biofilms and Pathogenesis.
e:
xiao-lin.tian@dal.caA new pheromone-guided antimicrobial peptide HP30 for targeted killing of
Streptococcus mutans
in
mixed-culture biofilms
Xiao-Lin Tian, Kayla Cyr, Xingxing Huang
and
Yung-Hua Li
Dalhousie University, Canada
Xiao-Lin Tian et al., Arch Clin Microbiol, 8:5
DOI: 10.4172/1989-8436-C1-003