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Ann Biol Sci, 2017
ISSN: 2348-1927
August 23-24, 2017 | Toronto, Canada
Annual Conference on
MICROBIAL PATHOGENESIS, INFECTIOUS DISEASE,
ANTIMICROBIALS AND DRUG RESISTANCE
Background:
One of the most important issues in healthcare
today is the development of bacterial resistance to
antibiotics which has created a generation of bacteria known
as multidrug resistant (MDR) bacteria. Due to antibiotics’
inability to treat these MDR bacteria metal and metal oxide
nanoparticles have been gaining interest as antimicrobial
agents. Among those, silver nanoparticles have been used
extensively as broad spectrum antimicrobial agents. Here
we describe the production and characterization of silver
nanoparticles made from the wood biopolymer lignin as a
reducing and capping agent with excellent antimicrobial
activity against MDR bacteria. We describe and compare
the productions of these particles both through a standard
heating procedure and through a microwave irradiation
procedure.
Methods:
The lignin-capped silver nanoparticles were
produced using a simple, one-pot synthesis method and
characterized by ultraviolet-visual spectroscopy, dynamic
light scattering, x-ray diffraction, and transmission
electron microscopy. These particles were then tested for
antimicrobial activity against clinical isolates of
S. aureus
700, P. aeruginosa, K. pneumoniae, A. baumannii, and E.
casseliflavus
. The bacteria were exposed to the particles
overnight in 96-well plates at increasing concentrations
(1–20 µg/mL), and their minimum inhibitory concentration
(MIC) was recorded for each bacterial strain.
Results:
Characterization of the lignin-capped silver nano
particles shows uniform spherical nanoparticles with a silver
core and a lignin coating with a diameter of 62±1.9 nm for
the standard heating and 42.03±0.39 nm for the microwave
synthesis, but the microwave method was much faster (10
min vs. 3 days). The MIC of the silver nanoparticles was ≤2.5
µg/mL for S. aureus 700 and
P. aeruginosa
, and ≤1 µg/mL for
all other tested strains.
Conclusion:
Lignin-capped silver nanoparticles can be
successfully produced using both standard heating and
microwave irradiation, and show very high antimicrobial
activity against a wide range of MDR bacterial strains.
Speaker Biography
Jason Asnis received his BEng in Chemical Engineering (2015) from McGill
University, Canada. He is currently an MSc student in the Häfeli Lab in the Faculty of
Pharmaceutical Sciences, at The University of British Columbia, Canada. In addition
to his research position, he is also the Social Director for the Pharmaceutical Sciences
Graduate Society.
e:
Jason.asnis@alumni.ubc.caThe production of lignin-capped silver nanoparticles with high antimicrobial activity against multidrug
resistant bacteria
Jason Asnis, Yael N Slavin, Horacio Bach, Katayoun Saatchi
and
Urs O Häfeli
University of British Columbia, Canada
Jason Asnis et al., Arch Clin Microbiol, 8:5
DOI: 10.4172/1989-8436-C1-003