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allied
academies
Ann Biol Sci, 2017
ISSN: 2348-1927
August 23-24, 2017 | Toronto, Canada
Annual Conference on
MICROBIAL PATHOGENESIS, INFECTIOUS DISEASE,
ANTIMICROBIALS AND DRUG RESISTANCE
F
ourteen
Lactobacillus plantarum
strains isolated from
various food sources and two different climatic regions
(Ireland and Pakistan) were genetically characterized
at subspecies level with
recA
gene based multiplex PCR
amplifications and pulsed-field gel electrophoresis. All
the strains were tested
in vitro
for functional probiotic
properties, which included the production of bacteriocin
against the major food borne pathogens (
L. innocua and L.
monocytogenes
), acid tolerance, survival in simulated gastric
juice, NaCl tolerance, bile salt hydrolaseactivity, andantibiotic
resistance. The genes encoding bacteriocin (plantaracin 423)
were identified from
L. plantarum
strains, and enzymes
sensitivity assays to protinease K and pepsin were tested.
Results of Genomic fingerprinting following ApaI digestion
revealed 10 distinctly different strains of PFGE patterns.
Antimicrobial screening revealed,
L. plantarum
AS-4, AS-6,
AS-8, AS-13 and AS-14 strains as the potential producers
of bacteriocin. The culture supernatants of these strains
expressed GIZ up to 12, 12, 14, 11 and 13 mm, respectively
against
L. innocua
and the 3932 Da molecular mass was
determined by using MALDI-TOF mass spectrometry along
with control (
L. plantarum
LMGP-26358) The positive
control, previously characterized plantaracin producer strain
LMGP-26358, also showed GIZ of 12 mm. On the other hand,
all the
L. plantarum
strains were active against a broad range
of microorganisms including
L. monocytogenes
DPC 6179,
Enterococcus facelis
5055 (LMG9737), E. coli DPC EC101,
Bacillus subtilis
LMG 8198,
Clostridium perfringens
LMG
10468,
Clostridium difficile
ATCC 42593 and
Staphylococcus
aureus
DPC 6867. Molecular characterization of these
isolates was performed by amplification of previously known
bacteriocin genes. Polymerase chain reaction analyses
revealed that plantaracin genes were present in the genome
of
L. plantarum
strains AS-4, AS-6, AS-7, AS-13 and AS-14
along with
L. plantarum
LMGP-26358 and for these bacteria
almost similar growth pattern of bacteriocin production
was observed. The loss of activity of 13 out of 15 strains
confirmed that the antimicrobial substance produced by
L.
plantarum
strains was indeed proteinaceous. All the strains
showed good
in vitro
functional potential and a significant
relationship was found between source of isolation and
functional score with promising probiotic potential. Some
of the desired characteristics were even better than those
of probiotic referenced strains. This study confirmed a high
heterogeneity in functional properties of the
L. plantarum
strains and provides insight into optimal screening strategies
Speakers Biography
Asma Manzoor has completed her PhD in Industrial Biotechnology from Govt. College
University Lahore with international training from Teagasc Moorepark, Dairy Product
Research Center, Fermoy Co. Cork Ireland. Her PhD project at Teagasc Moorepark,
was in the area of Comparative assessment of Probiotic attributes of
Lactobacillus
plantarum
strains of Ireland and Pakistan. She is currently working as Assistant
Professor at Institute of Biochemistry and Biotechnology University of the Punjab
Lahore.
e:
asmamanzoor33@hotmail.comJohar Ali is working as Assistant Professor at Rehman Medical Institute Peshawar,
Pakistan.
e:
johar_ali_1@yahoo.comComparative assessment of probiotic attributes of
Lactobacillus Plantarum
strains of Ireland and
Pakistan
Asma Manzoor
1
, Johar Ali
4
, Javed Iqbal Qazi
1
, Paul Ross
2, 3
and
Catherine Stanton
2, 3
1
University of the Punjab, Pakistan
2
Teagasc Food Research Center, Ireland
3
University College Cork, Ireland
4
Rehman Medical Institute Peshawar, Pakistan
Asma Manzoor et al., Arch Clin Microbiol, 8:5
DOI: 10.4172/1989-8436-C1-003