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Volume 4

Journal of Infectious Diseases and Treatment

ISSN: 2472-1093

Page 53

Euro Infectious Diseases 2018 &

Histopathology 2018

September 27-29, 2018

&

JOINT EVENT

September 27-29, 2018 Rome, Italy

5

th

International Conference on

Histopathology & Cytopathology

10

th

Euro-Global Conference on

Infectious Diseases

Interleukin-36α expression in vitiligo skin lesions and its correlation with disease pattern, activity and

severity

Laura del Barrio Prieto, Irene Beitia Rodriguez, Laura Martinez Codes, Mar Olmo Fernandez, M Jesús Lago Perez

and

Blanca Palomeque Castro

Hospital Del Henares, Spain

Introduction:

Macrocassettes are a very useful tool in the pathology laboratory. Currently, they are usually carried out

exclusively in radical prostatectomies. We are pioneers in Spain in using them for all types of tissues. It is not usually known

the adequate processing that allows the realization of good quality slides in any type of tissue. The fact that the pathologist can

visualize complete structures or larger lesions in a single slide improves efficiency.

Purposes:

To present our experience for more than ten years in the fixation, processing, inclusion and cutting of numerous

biopsies in macrocassettes (more than 4000) and show the advantages and disadvantages of the use of this technique. Currently,

we have launched a practical workshop for pathology technicians teaching these techniques. In this first edition of the workshop

we have full occupation from the participants and also we have received numerous emails from professionals from outside

Spain to get information to participate.

Equipment & Methods:

For more than ten years we have made macrocassettes of all kinds of tissues, bone, kidney, colon,

breast, brain with fixings and different processing times, processing the macrocassettes separated from the routine biopsies

(due to the time of permanence in each liquid), until obtaining optimal results in each type of tissue. We present our processing

protocols and abundant iconography of the process and the results.

Results & Conclusion:

The use of macrocassettes facilitates the diagnosis by the pathologist to be able to visualize structures

in a more complete way, provide more accurate tumor volumes and more appropriate assessment of surgical edges, automated

immunohistochemistry can be performed, collecting from the flotation bath the area that is needed for diagnosis. This

technique increases the profitability in the daily work by decreasing the number of cassettes. It’s recommended to incorporate

this technique to the routine only with a specific processor and qualified technicians in the handling of this type of cassettes.

lauradbp@hotmail.com

J Infec Dis Treat 2018, Volume 4

DOI: 10.21767/2472-1093-C1-003