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Notes:
Volume 4
Journal of Infectious Diseases and Treatment
ISSN: 2472-1093
Page 48
Euro Infectious Diseases 2018 &
Histopathology 2018
September 27-29, 2018
&
JOINT EVENT
September 27-29, 2018 Rome, Italy
5
th
International Conference on
Histopathology & Cytopathology
10
th
Euro-Global Conference on
Infectious Diseases
Clonal analysis and virulence-associated traits of native
Escherichia coli
from urethra of gilts and
natural/artificial pregnant sows
Sergio E Pasteris
1
, Andrea Torres Luque
1
, Alfredo A Martin
2
and
M V María C Otero
1
1
INSIBIO-UNT-CONICET, Argentina
2
FAZ-UNT, Argentina
Statements of the Problem:
The reproductive performance of sows is a key factor in the herd´s productivity. Urinary tract
infections (UTI) are a common problem in females, causing repeat breeding with a delayed return to estrus, which reduces the
animal´s welfare and the litter performance;
Escherichia coli
being associated to these infections. Diverse studies described a
unique microbiota in the UT in bitch. Others authors concluded that the composition of the UT bacterial communities could
have an important role in the health condition of the host.
Methodology:
We performed the isolation and clonal association (rep-PCR, Box and Eric primers) of E. coli from the urethral
microbiota of: healthy gilts-HG (n=9) and pregnant sows by natural breading-NB (n=11) or artificial insemination-AI (n=11).
Also, 12 virulence factors relevant for pyelonephritic strains were evaluated by PCR: hlyA, cnf, ibeA, iutA, kpsMT II, FimH,
papC, sfa/focD, afa/draBC, traT, agn43, csgA.
Findings:
Cultures revealed a slightly minor count (CFU/mL) for AI (3.7±0.59) group compared to HG (4.2±0.24) and NB
(4.3±0.44). However, there were no differences for E. coli isolation (CFU/mL): 1.45±1.36, 2.87±1.53 and 2.61±1.84, for AI, NB
and HG, respectively. The clonal analysis with both, Box or Eric primers, revealed a high similarity (>90%) between E. coli
isolates from different animal groups. Positive reaction was found for: FimH (76%), agn43 (92%), traT (32%) and csgA (72%),
these last ones showed a differential prevalence and were associated with E. coli from NB sows.
Conclusion & Significance:
These results indicate that the management conditions could affect the characteristics of the
urethral microbiota in sows and, therefore, the risk for urinary tract diseases.
Biography
Sergio E Pasteris has his expertise in the study of lactic acid bacteria (LAB) metabolism as well as in the isolation and evaluation of its beneficial properties to design
probiotics for amphibian culture. Taken into account the development of resistant bacteria, some LAB strains represent an alternative instead chemotherapeutics to
prevent epizootics in bullfrog systems breeding. Since bullfrog is a carrier of the etiological agent of chytridiomycosis, probiotics by using native LAB from bullfrog
skin are being developed to be applied during the ex situ breeding of endangered amphibian species.
pasteris@fbqf.unt.edu.arSergio E Pasteris et al., J Infec Dis Treat 2018, Volume 4
DOI: 10.21767/2472-1093-C1-003