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Volume 4
Journal of Infectious Diseases and Treatment
ISSN: 2472-1093
Page 47
Euro Infectious Diseases 2018 &
Histopathology 2018
September 27-29, 2018
&
JOINT EVENT
September 27-29, 2018 Rome, Italy
5
th
International Conference on
Histopathology & Cytopathology
10
th
Euro-Global Conference on
Infectious Diseases
Probiotics for amphibians: Advances in the selection of lactic acid bacteria for chytridiomycosis
control
Sergio E Pasteris, Maria V Niederle, Eduardo E Andrada Suarez, Sabrina I Volentini, Cesar E Ale
National University of Tucumán, Argentina
Statement of the Problem:
Chytridiomycosis is an amphibian skin disease caused by
Batrachochytrium dendrobatidis
(Bd) that
produces extinction around the world. Some amphibian-skin bacteria have been proposed as probiotic for chytridiomycosis
control but they excluded the lactic acid bacteria (LAB) group. Here in, we advanced in the selection of indigenous LAB
from bullfrog (considered as a Bd carrier) skin to design probiotic for application during the
ex situ
breeding of endangered
amphibians.
Methodology:
To determine the anti-Bd activity, co-culture assays between Bd strains (CFLT 159 from Brazil; AVS4 and AVS7
from Chile) and potentially LAB isolates were performed. Isolates previously shown exopolysaccharide (EPS) synthesis and/
or auto aggregation (AA) ability were evaluated for biofilm formation by using polyestyrene plates. Compatibility assays were
performed to evaluate the possibility to formulate a mixed probiotic.
Findings:
From 62 potentially LAB, 48 isolates had any anti-Bd activity. The 16s RNA sequence analysis allowed obtaining
97-99% of identity that matches with
Enterococcus
and
Lactobacillus
. Thus,
Enterococcus
sp. 90, 564, 747, 762;
Lactobacillus
sp. 10, 529, and
Enterococcus
gallinarum CRL 1826 (previously characterized) inhibited all the Bd strains. Three LAB isolates
exhibited low biofilm formation, while E. gallinarum showed moderate production. This ability was not always associated with
AA or EPS synthesis. The compatibility assays indicated that the LAB isolates could be included in mixed probiotic with the
exception of
Enterococcus
sp. 742 that was inhibited by E. gallinarum.
Conclusion & Significance:
E. gallinarum CRL 1826 resulted the best strain for a probiotic since it has many beneficial
properties: anti-Bd activity, AA, EPS synthesis, biofilm formation, medium hydrophobicity and GRAS properties according
to in vitro and in vivo tests (3-7). However,
Enterococcus
sp. 747 would maximize some probiotic properties of the CRL strain;
therefore, a mixed probiotic can be proposed.
Biography
Sergio E Pasteris has his expertise in the study of lactic acid bacteria (LAB) metabolism as well as in the isolation and evaluation of its beneficial properties to design
probiotics for amphibian culture. Taken into account the development of resistant bacteria, some LAB strains represent an alternative instead chemotherapeutics to
prevent epizootics in bullfrog systems breeding. Since bullfrog is a carrier of the etiological agent of chytridiomycosis, probiotics by using native LAB from bullfrog
skin are being developed to be applied during the ex situ breeding of endangered amphibian species.
pasteris@fbqf.unt.edu.arSergio E Pasteris et al., J Infec Dis Treat 2018, Volume 4
DOI: 10.21767/2472-1093-C1-003