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Volume 4
Journal of Infectious Diseases and Treatment
ISSN: 2472-1093
Page 41
Euro Infectious Diseases 2018 &
Histopathology 2018
September 27-29, 2018
&
JOINT EVENT
September 27-29, 2018 Rome, Italy
5
th
International Conference on
Histopathology & Cytopathology
10
th
Euro-Global Conference on
Infectious Diseases
Molecular genotyping of
Giardia duodenalis
in preschool children
Molouk Beiromvand
1
, Raziyeh Kasaei
1
, Ali Jelowdar
1
and
David Carmena
2
1
Ahvaz Jundishapur University of Medical Sciences, Iran
2
Carlos III Health Institute, Spain
G
iardia duodenalis
is an intestinal flagellated protozoan that infects humans and several animal species. Based on molecular
studies mainly targeting the small subunit (SSU) rRNA gene locus of the parasite, 8 assemblages (A to H) have been
identified in humans and other animal species. The aim of the current study was to evaluate the frequency and molecular
diversity of
Giardia
in pre-school children from rural and urban day care centers from Behbahan, Southwestern Iran. This
cross-sectional study was based on a concentration method for the microscopic detection of
G. duodenalis
in stool samples
of 450 children, aged 1-7 years. The survey was conducted from December 2015 to May 2016. PCR-methods targeting the
SSU rRNA and triose phosphate isomerase
(TPI)
genes of
G. duodenalis
were used for the identification and genotyping of
the parasite isolates. Based on sucrose flotation and microscopy techniques, 2.7% (12/450) of children were infected with G.
duodenalis, of which six (50.0%) were males and the other six (50.0%) were females. Overall, 91.7% (11/12) of the infections
were detected in children from rural areas. The SSU rRNA and TPI genes were amplified successfully in nine and eight,
respectively of the Giardia-positive samples at microscopy. Among the eight TPI sequences, assemblage A, sub assemblage AII,
was identified in five of the isolates. The sequences of the three remaining samples were untypable. Although no significant
statistical difference between genotype and clinical symptoms was found, five out of the eight isolates identified as assemblage
A were obtained in asymptomatic children.
Giardia
infections were more prevalent in children from rural day care schools and
the predominant assemblage was A, sub-assemblage AII. The higher prevalence of giardiasis in rural areas might be related
to differences in personal hygiene habits, parents’ education level, source of drinking water and inadequate hygienic toilet
facilities in rural areas.
Biography
Molouk Beiromvand has completed her PhD at Tehran University of Medical Sciences, Tehran, Iran. She is an Assistant Professor in the Department of Parasitology,
School of Medicine at Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Beiromvand-m@ajums.ac.irMolouk Beiromvand et al., J Infec Dis Treat 2018, Volume 4
DOI: 10.21767/2472-1093-C1-003