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Volume 4 Journal of Infectious Diseases and Treatment
ISSN: 2472-1093
Page 39
Euro Infectious Diseases 2018 &
Histopathology 2018
September 27-29, 2018
&
JOINT EVENT
September 27-29, 2018 Rome, Italy
5th International Conference on Histopathology & Cytopathology
10th Euro-Global Conference on Infectious Diseases
Molecular association of lncRNA-uc003wbd and lncRNA-AF085935 expression with a genetic variant
profile in Egyptian patients with hepatocellular carcinoma and HBV
Tarek Mohamed Kamal Mohamed Metawie, Shohda Assem El-Maraghy, Dina Sabry Abdel Fatah and Noha Ali Mehana
Cairo University, Egypt
Hepatocellular carcinoma (HCC) was among the most common solid tumors, rated third in cancer-related mortality
worldwide. The burden of HCC has been increasing in Egypt with a doubling in the incidence rate in the past 10 years.
This has been attributed to several biological (e.g. hepatitis B (HBV) and C (HCV) virus infection) and environmental
factors. HBV infection is of particular interest, for its coherent distribution with the HCC prevalence. Thus, new diagnosis
measures and targeted treatments for HCC and HBV are in need. Long non coding RNAs (lncRNAs) are dysregulated in
different cancers and have critical roles in various biological processes such as HULC and MALAT1 may participate in HCC
development and progression. Besides, considerable studies have investigated the effects of lncRNAs genetic variations on
cancer susceptibility. Through this study, we aimed at exploring the expression profile and the potential clinical value of
two lncRNAs (lncRNAuc003wbd and lncRNA-AF085935) in differentiating HCC from both HBV patients and the healthy
specimens and their associations with single nucleotide polymorphisms (SNPs) in HULC and MALAT-1 and the susceptibility
to HBV chronic infection and HCC. Serum samples were extracted from 70 HBV patients, 70 HCC patients, and 70 healthy
controls. The level of serum lncRNA-uc003wbd and lncRNA-AF085935 of all the subjects were assayed by quantitative real-
time reverse transcriptase polymerase chain reaction (qRT-PCR). Moreover, we have genotyped two SNPs, rs7763881 in HULC
and rs619586 in MALAT1, in all subjects to test the association between the two SNPs and susceptibility to HCC and HBV
chronic infection. The level of serum lncRNA-uc003wbd and lncRNA AF085935 was significantly upregulated in HCC patients
and HBV patients compared with that in normal controls. The variant genotypes of rs7763881 were significantly associated
with decreased HCC risk. Similarly, variant genotypes of rs6682925 were associated with non-significant decreased HCC
risk compared with the wild-type AA genotype. However, no significant association was found between the two SNPs and
HBV clearance. In conclusion, our results showed that both lncRNAuc003wbd and lncRNA-AF085935 are able to be potential
biomarkers for HCC and HBV screening as well that SNP rs7763881 in lncRNA HULC was significantly associated with the
decreased susceptibility to HCC in HBV persistent carriers.
Biography
Tarek Mohamed Kamal Mohamed Metawie is a Professor of Biochemistry in the Faculty of Pharmacy, Cairo University. He has completed his PhD in Pharmaceutical
Sciences in 1984; MSc in Pharmaceutical Sciences in 1979; BSc in Pharmaceutical Sciences in the Faculty of Pharmacy at Cairo University in 1976. Professional
experience: Instructor; 1976, Lecturer Assistant; 1980, Lecturer, 1984; Assistant Professor, 1989; Professor, 1994; Head of the Department of Biochemistry, Faculty
of Pharmacy, and Cairo: - 2008-2014.
Tarek Mohamed Kamal Mohamed Metawie et al., J Infec Dis Treat 2018, Volume 4
DOI: 10.21767/2472-1093-C1-003