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Notes:
Volume 4
Journal of Infectious Diseases and Treatment
ISSN: 2472-1093
Page 40
Euro Infectious Diseases 2018 &
Histopathology 2018
September 27-29, 2018
&
JOINT EVENT
September 27-29, 2018 Rome, Italy
5
th
International Conference on
Histopathology & Cytopathology
10
th
Euro-Global Conference on
Infectious Diseases
Proteomic characterization of
Haemaphysalis longicornis
saliva and determination of saliva-host
interaction during blood-sucking periods
Myungjo You
1
, Mohammad Saiful Islam
2
, Md. Khalesur Rahman
1, 2
and
ItabajaraDaSilvaVazJr
3
1
Chonbuk National University, Republic of Korea
2
Hajee Mohammad Danesh Science and Technology University, Bangladesh
3
Federal University of Rio Grande do Sul, Brazil
Abstract:
H.longicornis
saliva proteomic profile, role of histamine-antihistamine and cytokines interection with host were
design and performed in this work.
Methods:
For salivary protein LC-MS/MS was performed. Histamine and antihistamine were administered at different interval
to see its effect on detachment during attachment and fast feeding stage, respectively. Histamine concentration was measured
by ELISA and differences in expression of genes related to immune response were measred by qPCR.
Results:
A total of 135 tick salivary proteins were identified of which 30 proteins were found exclusively in fully engorged
nymph saliva, 74 in fully engorged adult females, and 31 were detected in both stages. Ticks exhibited a higher detachment rate
(30.1%) 12 hours after histamine application during attachment stage and antihistamine injection (25.44%) at 96 hours during
fast feeding stage. There is an average increases in body weight were observed for histamine treated group and decrease in body
weight was observed in antihistamine treated
H.longicornis
in compare to control. Blood histamine concentration during fast
feeding stage was significantly higher in compare to attachment and no-infested groups. Gene expression of TNFα, IFNγ were
significantly (P<0.05) decrease in all stages of blood sucking both in nymph and adult ticks, while expression of IL-4, IL-6 and
IL-10 were increased 1.3 to 7 folds in adult infesteted rabbits in comapre to non-infested with the exception of IL-6 in nymph
infested rabbit. IL-6 significantly decreased in nymph infested rabbits and IL-2 was not expressed in either nymph or adult
H.longicornis
treated rabbit. These data can provide a deeper understanding to the biology of H. longicornis.
Biography
Myungjo You’s lab has focused on unveiling how hosts response to hard tick infection. They have used various infectious models to prove host responses upon tick
infection. In recent, You’s lab has found the saliva from nymph and adult females of Haemaphysalis longicornis: a proteomic study (Parasite & Vectors). Moreover,
You’s lab is expert on tick and tick-borne disease.
tick@chonbuk.ac.krMyungjo You et al., J Infec Dis Treat 2018, Volume 4
DOI: 10.21767/2472-1093-C1-003