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Volume 4
Journal of Infectious Diseases and Treatment
ISSN: 2472-1093
Page 69
Euro Infectious Diseases 2018 &
Histopathology 2018
September 27-29, 2018
&
JOINT EVENT
September 27-29, 2018 Rome, Italy
5
th
International Conference on
Histopathology & Cytopathology
10
th
Euro-Global Conference on
Infectious Diseases
3-hydroxy-3-methylglutaryl coenzymeAreductase (HMGR) from
Leishmania
donovani is indispensible
for the parasites as depicted by gene knockdown studies
Sushma Singh
National Institute of Pharmaceutical Education and Research (NIPER), Punjab, India.
L
eishmania
sis is one of the most diverse and complex of all vector borne diseases which are transmitted through the bite
of infected female Phlebotomine sand flies. Existing anti
leishmania
l drugs are associated with toxicity, resistance issue,
high prices and lack of quality during administration and long treatment length. Sterols are the important components of the
cell membrane which are essential for cellular functions and maintain the cell structure. In humans, HMGR is a rate limiting
enzyme of cholesterol biosynthesis and is a trans-membrane glycoprotein. In
Leishmania
, however HMGR exists as a soluble
protein involved in ergosterol biosynthesis. The sterol biosynthetic pathway in
Leishmania
is considered to be an important
drug target. In the present study, null mutants of LdHMGR were generated by homologous recombination and mutants were
confirmed by PCR, western blotting and enzyme activity. The ergosterol level was quantified by HPLC method and found that
the SKO LdHMGR parasite ergosterol level was low when compared to WT parasite and 99.14% reduction was observed in
DKO LdHMGR. Growth curve analysis of SKO LdHMGR was found to be less with rapid reduction in growth rate of the DKO
LdHMGR promastigotes as compared with WT promastigotes. Infectivity analyses showed decreased percentage of host cell
line infection by null mutants of LdHMGR as compared to SKO LdHMGR and WT promastigote parasite. This data suggests
that HMGR plays an essential role in growth and infectivity of parasite and it is an essential enzyme and can be utilized for
future anti
leishmania
l chemotherapeutic intervention.
sushmasingh@niper.ac.inJ Infec Dis Treat 2018, Volume 4
DOI: 10.21767/2472-1093-C1-003