27 / 33
Volume 4
Journal of Infectious Diseases and Treatment
ISSN: 2472-1093
Page 63
Euro Infectious Diseases 2018 &
Histopathology 2018
September 27-29, 2018
&
JOINT EVENT
September 27-29, 2018 Rome, Italy
5
th
International Conference on
Histopathology & Cytopathology
10
th
Euro-Global Conference on
Infectious Diseases
In vitro
and
In vivo
anti-malarial activity of extracts from
Terminalia mantaly
(combretacéae)
Mariscal B. Tchatat Tali
1
, Cedric D. Jiatsa Mbouna
1
, Lauve R. Tchokouaha Yamthe
2
, Patrick V. Tsouh Fokou
1
and
Fabrice F. Boyom
1
1
Department of Biochemistry, University of Yaoundé, Cameroon
2
Institute for Medical Research and Medicinal Plants Study, Cameroon
T
he emergence of resistance of malaria parasite to available drugs highlight the urgent need to develop new efficient, safe
and affordable drugs. In Cameroon, medicinal plants such as T. mantaly are used in traditional medicine for the treatment
of malaria and have been playing an important role in the fight against malaria especially in rural community. However, their
efficacy are still to be validated. This work aimed to investigate the
in vitro
and
in vivo
antimalarial potency of extracts from
Terminalia mantaly. Extracts from stem barks, leaves and roots of T. mantaly were macerated in water and methanol. The
susceptibility of red blood cells to the extracts was using the MTT assay. The antiplasmodial activity was performed on the
W2 strain of Plasmodium falciparum. Prior to the curative test, acute toxicity of the promising aqueous stem bark extract was
assessed in mice at a dose of 2,000 mg/kg/bw. Mice infected with P. berghei MRA 406 strain were treated with the promising
extract at doses of 100, 200, 400mg/kg. Their parasitemia were monitored as well as their hematological, biochemical and
histological parameters. Extracts did not shown any cytotoxicity on erythrocytes at up to 1mg/mL. Out of the six extracts
tested, two presented
in vitro
antiplasmodial activity with IC50 of 0.809 and 2.203 µg/ml respectively. The acute toxicity assay
of the aqueous extract from stem bark revealed 50% lethal dose (LD50) higher than 2000mg/kg per body weight. The curative
test showed an effective dose that reduce 50% of parasitemia (ED50) of 69.50mg/kg with no significant effect on biochemical,
hematological and histological parameters. The results from this investigation support the traditional usage of T. mantaly and
suggest that stem bark of T. mantaly could be potential source of compounds with anti-malaria activity. However, further
investigations are needed to characterize active principles.
b.tchatat@yahoo.comJ Infec Dis Treat 2018, Volume 4
DOI: 10.21767/2472-1093-C1-003