euro-infectious-diseases-histopathology-2018-posters

Volume 4

Journal of Infectious Diseases and Treatment

ISSN: 2472-1093

Page 57

Euro Infectious Diseases 2018 &

Histopathology 2018

September 27-29, 2018

JOINT EVENT

September 27-29, 2018 Rome, Italy

International Conference on

Histopathology & Cytopathology

Euro-Global Conference on

Infectious Diseases

A study on genomic diversity of norovirus using deep sequencing approach

InSun Joo, JeongSu Lee, EunSook An, SiYeon Ju

and

MinHee Jeong

National Institute of Food and Drug Safety Evaluation, Korea

study on genomic diversity of norovirus using deep sequencing approach: Norovirus (NoVs) are the leading cause of

epidemic and sporadic gastroenteritis outbreaks worldwide affecting across all age groups, responsible for approximately

90% of all outbreak of viral gastroenteritis. Each year, mortality due to NoV is estimated at 570-800 in the United States, with

approximately $777 million in health-care costs. NoV, a genus within the

Caliciviridae

family, is small non-enveloped virus

with a positive single-stranded RNA genome of 7.5-7.7 kb organized into three open reading frames (ORFs). ORF1 encodes

six non-structural proteins, including RNA dependent RNA polymerase (RdRP). ORF2, ORF3 encode VP1 and VP2 capsid

proteins. Generally, the genome of RNA virus has been known to change constantly from mutational event and revealed novel

variant. In the previous reports, the NoV GII.4 strains had been known to evolve at a rate of 4.3-9.0X10-4 mutations per site

per year and to share a most recent common ancestor in the early 1980s. The goal of this study was to analyze NoV complete

sequence and detect variants using next generation sequencing (NGS) method. Sequence reads of NoV were constructed

to GII.4 whole genome sequence and were assembled with reference genome. In phylogenetic analysis, the sequence was

clustered with 2006b variants. Moreover, VP1 (i.e. capsid protein) were revealed that the amino acid sequence altered three

sites in hyper variable domains, and it led to changes in protein structure. Continued molecular studies of NoV by NGS,

including approaching of complete genome, is important for monitoring emerging strains in our strategy to prevent of NoV

outbreaks in Korea.

J Infec Dis Treat 2018, Volume 4

DOI: 10.21767/2472-1093-C1-003