Studies on the phytochemistry, antibacterial activity and green synthesis of nanoparticles using Cassia tora l. against amphicillin resistant bacteria

The field of nanotechnology is one of the most active areas of research in modern materials science and technology. An ecofriendly green mediated synthesis of inorganic nanoparticle is a fast growing research in the limb of nanotechnology. The present study synthesis from 1 mm AgNO3 solution through the leaf extract of Cassia tora L. as reducing as well as capping agent. Nanoparticles were characterized using UV –vis absorption spectroscopy’s green synthesized silver nanoparticles showed the antibacterial against the isolated microorganisms. Escherichia coli, Pseudomonas sp. and Salmonella sp. were isolated from clinical specimen. Phytochemical analysis of aqueous extracts of Cassia tora L. showed the presence of anthraquniones, carbohydrates, glycosides, cardiac glycosides, amino acid, phytosterols, fixedoils and fats, phenolic compounds, tannins, flavonoids, steroids and saponins. The silver nanopaticles was characterized by using UV –visible Spectrophotometer at the range of 200-800nm. Absorption spectrum (200- 800nm) at the beginning of the reaction showed three distrinct peaks , centering around 220, 280 and 430nm. The absorption spectrum showed increase in intensity over a broad spectrum in the range of 350-600nm within 10min of contact time while the remaining part of the absorption spectrum in the range of 350-600nm continued to increase with increasing incubation time till 60min. Each antibiotic resistant microorganism differs in the formation of zone of inhibition representing their sensitiveness to silver nanoparticles extracts of Cassia tora.

Author(s): Sathya, A* and V. Ambikapathy

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