Phyto-chemical Screening Analysis and Isolation of Bioactive Components in Fenugreek using GC-MS Study

The present study has focused on Fenugreek (Trigonella foenum-graecum.L) having antibiotic properties of antipyretic, anthelmintic, antileprotic, antibronchitic, carminative and anti-diabetic properties. The botanical name of the fenugreek is Trigonella foenum-graecum.L. The main aim of this study is to carry out phyto-chemical screening tests in the extract of Trigonella foenum-graecum.L to evaluate alkaloids, tannins, sterols, saponins, flavanoids, carbohydrates, glycosides, new anti-diabetic compound and various poly-phenol compound from plants available in our locality. As such, an attempt has been made to isolate the anti-diabetic compounds from the seed of Trigonella foenum-graecum.L. In this study, the solvent extraction study followed by using GC-MS Clarus SQ 8C Perkin Elmer to purify the compounds. Then, fenugreek seed powder extract analysis was conducted that revealed the presence of twenty one peaks of GC-MS chromatogram. Six chemical constituents were identified by GC-MS analysis. The major constituents were 4-t-Butyl-2-[4-nitrophenyl] phenol, n-Hexadecanoic acid, 9,12-Octadecadienoic acid (Z,Z), 9- Octadecadienoic acid (E), Octadecadienoic acid and Hexadecanoic acid, 2-hydroxy-1 which possess many antioxidant and biological activities. It can be applied in other food industries as a natural antioxidant instead of synthetic antioxidants that can be utilized for the growth of conventional medicines and further research needs to evaluate novel active compounds from the medicinal plants which may be produced in an enhanced way to treat many incurable diseases related to diabetes.

Author(s): M.Chenganmal*1 and C.Narayanan2

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