Micropropagation of Asparagus racemosus (Shatavari)

Asparagus racemosus (Shatavari) is a plant with immense medicinal values. It is reported to be recalcitrant to tissue culture practices. Sterile cultures were obtained when the explants were treated with (0.5%) Bavestine, Indophyl and 8HQC for 7 min and HgCl2 for 5 min.The present study on micropropagation of Asparagus racemosus through nodal explants showed better result in BAP (2 mg/l) along with IAA (0.2 mg/l) combination. Treatment of 0.5 % Bavestine, indophyl and 8HQc for 7 min along with 0.1% HgCl2 for 5 min gave 80% contamination free culture.Combination of BAP (2 mg/l) along with IAA (0.2 mg/l) was the best for shoot proliferation and gave 5 shoots on the 20th day of inoculation.Maximum rooting was obtained in MS basal media incubated in the dark.

Author(s): Abha Singh, O. P. Verma* and E. P. Koshy

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