In-vitro direct regeneration from nodal explants of Toddalia asiatica (L.) Lam.

Micropropagation is an important biotechnological tool for select, multiply and conserve the critical genotypes of medicinal plants. It also offer an integrated approach for the production of standarized quality phytopharmaceutical through mass-production of consistent plant material for physiological characterization and analysis of active ingredients. The present study was developed an efficient micropropagation protocol by using nodal explants of T. asiatica. Explants are surface sterilized and inoculated into culture medium with different concentrations of plant growth regulators. Initially, explants were cultured on Murashige and Skoog’s (MS) medium supplemented with different concentrations of BAP (0.5. 1.0, 1.5, 2.0, 2.5 & 3.0 mg/l) and in combinations with IAA. Highest number of shoots was obtained from nodal explants cultured on MS medium containing 2.5 mg/l BAP in combination with IAA (0.5 mg/l).The shoots developed through in-vitro regeneration were rooted on half strength MS media containing IBA and NAA and the highest number of roots was observed on MS medium with IBA (1.5 mg/l). The highest shoot sprouting efficiencies (95 %) and 38.1 shoots per explants, the highest shoot length were obtained about7.2 cm.

Author(s): S. P. Anand*, G. Velmurugan and A. Doss

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