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In-vitro antioxidant and hemorrhoidal potential of hydroethanolic leaf extracts of Bergenia ciliata, Bergenia ligulata and Bergenia stracheyi

The antioxidant properties of hydroethanolic extract of Bergenia species namely Bergenia ciliata, Bergenia ligulata and Bergenia stracheyi were analyzed using ten antioxidant assays. The absorbance for ferric thiocyanate assay and Thiobarbituric acid (TBA) assay was 0.058 nm and 0.082 nm. The half-maximal inhibitory concentration (IC50) for Phosphomolybdenum assay, Nitric oxide radical inhibition assay, ABTS radical scavenging assay, Hydroxyl radical scavenging activity, Peroxynitrite scavenging assay, Singlet oxygen scavenging activity, Hypochlorous acid scavenging activity, Deoxyribose assay was 110.71 μg/ml, 64.79μg/ml, 19.45 μg/ml, 1.931μg/ml,39.25 μg/ml, 30.53 μg/ml, 76.86 μg/ml, 5.661μg/ml respectively. This experiment presents total phenolics content (0.081 mg GAE/g) and flavonols content (0.079 mg TAE/g). A non-significant relationship between antioxidant capacity and total phenolics content, total flavonols content indicates stoichiometry of reaction, between antioxidant compounds in the extracts and the various radical. This may be inferred as a reason for the difference in their scavenging potential.

Author(s): Km. Ruby, Suman Sharma, Rajani Chauhan* and Jaya Dwivedi

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