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Direct Shoot Organogenesis from Juvenile Cotyledon of Jatropha podagrica Hook

Jatropha podagrica Hook is one species of the genus Jatropha. The Roots and vegetative parts of J. podagrica contain biologically active compounds and its seeds comprise high oil content. There is urgent need to develop mass propagation of J. podagrica by in vitro cultures. The objective of this research was therefore to induce direct shoots from juvenile cotyledon. Seeds were grown in vitro for 12 days and juvenile cotyledons excised for direct shoot organogenesis. MS (Murashige & Skoog) solid medium supplemented with 2.22-13.32 μM BAP, 0.49 μM IBA and 2.27-6.81 μM TDZ enriched with 3% (w/v) sucrose and 0.8% agar were used. The best shoot induction (3.00 ± 0.89 shoots per explant) was achieved after 6 weeks at a combination of 2.22 μM BAP, 0.49 μM IBA and 4.54 μM TDZ. The elongated renewed shoots were transferred to root induction medium containing half strength MS salts supplemented with either indole 3-butyric acid (IBA) or indole acetic acid (IAA). Best rooting was achieved on the medium supplemented with 9.8 μM IBA. Rooted plantlets were then acclimatized under greenhouse and showed 60% survival rate.

Author(s): Elias Zerai Tsegay, Siripong Premjet, Anupan Kongbangkerd and Duangporn Premjet*

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