Direct regeneration of Agele marmelos (L.) Corr. from shoot tip explants through in vitro studies

An efficient in vitro propagation protocol through shoot tip explants culture was developed for Agele marmelos (L).corr.shoot tip explants of in vitro grown 1 month old seedlings were cultured on MS basal medium supplemented with BAP and combination with IAA were differentiated into shoots directly. The shoots could be easily multiplication on MS Medium supplemented with 2.0mg/l (BAP) along with IAA (0.1-1.0mg/l). The establishment efficiency of in vitro from plantlets in pots containing a mixture of sand: vermiculate soil (1:1) was more than 90%. This rapid regeneration protocol an average of 15 plantlets was able to produce from single set of treatment.

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