A comparative analysis of genetic diversity across certain Mungbean
and Urdbean cultivars of West Bengal, using ISSR markers

Swati Das (Sur); Surya Shekhar Das; Parthadeb Ghosh

Abstract

A comparative analysis of genetic diversity across certain Mungbean and Urdbean cultivars of West Bengal, using ISSR markers

The objective of the present study was to prepare a comparative account of the extent of genetic diversity and genetic relationships among the 9 Vigna varieties based on DNA data. A total number of 10 ISSR primers that produced polymorphic and reproducible fragments were selected to amplify genomic DNA of the genotypes under investigation. Ten primers amplified a total number of 624 bands under 104 loci across nine genotypes with average of 10.4 loci / primer. The cultivars exhibited an overall 76.92% polymorphism. The value of Jacaard’s similarity coefficient ranged from 0.566 to 0.793. Panna showed the highest genetic diversity among all the five cultivars of mung bean, but was surprisingly and interestingly lower than any of the four cultivars of urd bean. The urd bean variety Kalindi seems to be genetically the closest one to the mung bean varieties studied. In comparison to other cultivars, Gautam showed the most genetic variability, suggesting utilization of this genotype over others for breeding programme and in transferring important characters into different cultivars. Overall, a high degree of genetic diversity among the 9 cultivars was recorded, which can be used for crop improvement especially keeping in mind the fact that mung bean has been reported to cross with a number of Vigna species when it is used as a female parent. The information generated from this study will be helpful to identify the germplasms, know their phylogenetic relationships and above all to construct an interspecific hybridization strategy.