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Page 35
Journal of Clinical Nutrition & Dietetics
ISSN:2472-1921
3 r d W o r l d C o n g r e s s o n
Nutrition, Dietetics
and Nutraceuticals
F e b r u a r y 2 5 - 2 6 , 2 0 1 9
P r a g u e , C z e c h R e p u b l i c
World Nutrition 2019
Psoriasis is a common chronic inflammatory disease. Under an inflammatory
stimulus, epidermis can convert arachidonic acid to prostaglandin E2
(PGE2), a pro-inflammatory mediator which can elevate the cellular levels of
cyclic nucleotides promoting psoriasis pathogenesis. Adherence to prescribed
drugs might be a problem in some patients due to side effects associated,
so finding an alternative natural treatment is of great interest. Due to the
anti-inflammatory properties related to olive ( Olea europaea L.) leaves, the
objective of this work was to characterize the bioactive components of
Xorialyc®, an olive leaves extract standardized to ortho-diphenols and luteolin-
7-o-glucoside, and test its anti-inflammatory action compared to other similar
extracts. Identification of Luteolin-7-o-glucoside was accomplished by high
performance liquid chromatography, while the characterization of ortho-
diphenol content was carried out by colorimetric determination as catechin
monohydrate. To determine the anti-inflammatory activity the production of
PGE2 was measured in lipopolysaccharide and interferon γ-stimulated murine
macrophages, RAW264.7 cells, in the presence or absence of different doses
of Xorialyc®, Diclofenac or other olive leaves extracts. More than 1 mg/g of
Luteolin-7-O-glucoside was quantified in Xorialyc® sample at 355 nm. The
total ortho-diphenolic content was higher than 30% of dry basis. All extracts
inhibited the release of PGE2 in a dose dependent relation, being Xorialyc®
the most active (p-value ≤0.05). Compared with Diclofenac, Xorialyc® induced
higher inhibition of PGE2 release at lower doses (p-value ≤0.05).The high levels
of ortho-diphenols and Luteolin-7-O-glucoside at Xorialyc®may be responsible
for the higher inhibition of PGE2 release compared to other commercial olive
leaves extracts, being at lower doses more active than Diclofenac. These
findings may help in the search of more natural anti-psoriatic treatments as an
alternative to the pharmacological ones that, in general, are related to higher
side effects.
Biography
Daniel Gonzalez-Hedstrom has obtained his Bachelors’ in
Biochemistry in 2016 at the Universitat de les Illes Balears
(Spain). After completing the Bachelors’ Biochemistry, he
obtained his Masters’ in Pharmacological Research at the
Universidad Autonoma de Madrid (Spain), where he carried out
his Master thesis about the cardiovascular insulin resistance
in an experimental model of childhood obesity in rats. He is
doing his PhD in Pharmacology and Physiology at the Research
& Development department of the company, Pharmactive
Biotech Products S L in collaboration with the research group of
Dr Miriam Granado, who protects the doctoral thesis, under the
Doctorado Industrial Fellowship by the Comunidad de Madrid.
His work is focused in the development of new plant-based
nutraceuticals and tests its functionality in vitro and in vivo.
He has recently published 3 papers in Q1 journals and he has
participated in many different scientific congresses.
Anti-inflammatory properties of the olive leaf extract
Xorialyc® (Olea europaea L.)for psoriasis treatment
Daniel Gonzalez-Hedstrom1,2, P Almodovar2 , I Jarama2, A
Salamanca2, A M Inarejos-Garcia 2
1Universidad Autonoma de Madrid, Spain
2Pharmactive Biotech Products S.L., Parque Científico de Madrid, Spain
Daniel Gonzalez-Hedstrom et al., J Clin Nutr Diet 2019, Volume: 5
DOI: 10.4172/2472-1921-C1-005