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Journal of Clinical Nutrition & Dietetics
ISSN:2472-1921
3
r d
W o r l d C o n g r e s s o n
Nutrition, Dietetics
and Nutraceuticals
F e b r u a r y 2 5 - 2 6 , 2 0 1 9
P r a g u e , C z e c h R e p u b l i c
World Nutrition 2019
T
he differentiation of monocytes into macrophages is a fundamental step
for the development of atherogenesis and atherosclerosis. Macrophages
participate in lipoprotein accumulation giving rise to foam cells filled with
lipid droplets. Several studies seem to suggest an atheroprotective effect of
phenolic compounds through a modulation of lipid metabolism. The objective
of the present study is to evaluate the role of caffeic acid (CA) and chlorogenic
acid (CGA) in counteracting lipid accumulation in a model of monocytes (THP-
1) differentiated to macrophages.THP-1-derived macrophages were incubated
for 24 h with fatty acids (500 μmol/L oleic/palmitic acid, 2:1 ratio) and phenolic
acids (CA and CGA, as single compounds or mix) at different concentrations
(0.03, 0.3 and 3 μmolL-1). Lipid accumulation was quantified with the
fluorescent dye Nile red. The fluorescence (excitation: 544 nm, emission:
590 nm) was measured in a fluorescence spectrophotometer and the fold
increase compared to the control (without fatty acids) was calculated. Data
were analysed by one way ANOVA. ANOVA revealed a significant increased
lipid accumulation following the fatty acids exposure (p<0.0001). The mix of
CA+CGA significantly reduced lipid accumulation at all concentrations tested
(-27.5%, -32.0%, -23.4%, respectively at 0.03, 0.3 and 3 μmolL-1; p<0.01).
Conversely, no effect was observed following the incubation with the single
compounds. Although preliminary, the results seem to indicate a potential
effect of CA+CGA, but not of the single phenolics, in counteracting lipids
accumulation in THP-1-derived macrophages. The effects were observed at
physiological relevant concentrations. Ongoing experiments will be useful to
confirm the results obtained and to clarify the potentials mechanisms of action
involved in the prevention of the atherogenesis process.
Biography
Cristian Del Bo has completed his PhD in Experimental
and Clinical Nutrition and Postdoctoral studies in Human
Nutritionfrom the University of Milan. He has completed an
Internship at the Antioxidants Research Laboratory, Jean Mayer
USDA Human Nutrition Research Center on Aging at Tufts
University (Boston, MA), at the Department of Food Science
and Human Nutrition of the University of Maine (Orono, ME)
and at the Department of Public Health of the University of
Copenhagen. He is a Research Fellow in Human Nutrition at
the University of Milan. He has published more than 30 papers
in peer-reviewed journals. He is on the Editorial Board of the
International Journal of Food Sciences
and
Nutrition
and Guest
Editor of the Special Issue “Dietary Bioactives and Human
Health” for the journal
Nutrients.
cristian.delbo@unimi.itEffect of caffeic and chlorogenic acid in the modulation of
lipid accumulation in THP-1-derived macrophages
Cristian Del Bo, Mirko Marino, Massimiliano Tucci, Patrizia
Riso and Marisa Porrini
University of Milan, Italy
Cristian Del Bo et al., J Clin Nutr Diet 2019, Volume: 5
DOI: 10.4172/2472-1921-C1-005