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Structural Biology 2018
Volume: 4
Biochemistry & Molecular Biology Journal
Page 62
March 15-16 2018
Barcelona, Spain
10th Edition of International Conference on
Structural Biology
Introduction: MicroRNAs (miRNAs) are small non-coding RNAs,
approximately 22 nucleotides in length that mediate translational
repression of target messenger RNA (mRNA) transcripts.
MiRNAs play a key role in cellular differentiation and proliferation,
regulation of cellular metabolism, and are involved in the initiation
andprogressionof cancer.Therefore, comparingof two states can
illustratewhich genes cause the cancer initiation and progression.
RNA-seq is the best high throughput way to study the expression
level of genes and microRNAs entirely in cells with different
states. Some genes are overexpressed and some of them are
down-regulated in different physiological conditions or diseases
especially in cancer. The main purpose of the current study is to
figure out the specific miRNAs between all the microRNAs and
analyze their target genes to find out the connection between the
dysregulator miRNAs and CRC-related pathways.
Materials & Methods: In this study, the microRNA expression
level of 1061 malignant colorectal cancer and 30 normal samples
were compared using TCGA database and python programing
language. Fold changes were calculated for high-grade samples
related to normal ones. From total 1882 microRNAs, 10 top
differentially-expressed miRNAs that had significant p values
(p<0.05) were chosen for further analyses. Then 3000 in-common
target genes, by using microRNA, miRwalk and miRmap online
tools, were selected. After that Mirfocus and Diana online tools
were intended to analyze miRNA-target gene pathways and the
related miRNA annotations. Finally, KEGG database and Panther
online tool were used to illustrate which signaling pathways are
important in colorectal cancer based on microRNA function.
Results & Conclusion: Analysis of top differentially expressed
microRNAs including hsa-miR-9-3, 9-1, 9-2, 200a, 1307, 191,
210, 192, 17, 1511 and hsa-miR-27a and their in-common target
genes indicated that Wnt, cadherin, integrin and CCKR signaling
pathways are more activated in malignant colorectal cancer than
normal selected samples. Thus, these dysregulated miRNAs may
have a key role in CRC initiation and progression by targeting
some of the most important genes in CRC-involved signaling
pathways. However, more experimental studies are needed to
elucidate exact mechanism of the miRNAs function in CRC-
related pathways.
Bioinformatic analysis of gene expression profiles of CRC
samples by RNA-seq data set
Atena Yasari, Hamed Dabiri and Bahram Mohamad Soltani
Tarbiat Modares University, Iran
Biochem Mol biol J, Volume 4
DOI: 10.21767/2471-8084-C1-009